ABO Blood Type Compatibility Experiment: Understanding Blood Transfusions, Study notes of Personal Health

Instructions for an experiment using the red cross abo blood type game to demonstrate blood type compatibility in transfusions. Students will label cups with different blood types, use pipettes to mix 'blood' samples, and observe color changes to determine safe and unsafe transfusions. Background information on blood types and their prevalence in the population, as well as instructions for testing different patient types with various blood types. The experiment aims to help students understand the importance of blood type compatibility in transfusions and the limitations of blood donation.

Typology: Study notes

2021/2022

Uploaded on 09/27/2022

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ABO Blood Type Game
The following lesson is adapted from the Red Cross ABO Blood Type Game
Materials
8 3.5 oz cups labeled “ A”
8 3.5 oz cups labeled “ B”
8 3.5 oz cups labeled “ AB”
8 3.5 oz cups labeled “ O”
32 pipettes
1 250 ml bottle water plus 15 drops red food coloring, labeled “A”
1 250 ml bottle water plus 10 drops blue food coloring, labeled “B”
1 250 ml bottle water plus 7 drops red + 5 drops blue food coloring, labeled “AB”
1 250 ml bottle water, labeled “O”
15 12-well plates
30 worksheets
Color Concentration for food coloring:
I use the ratio of 5 drops of food coloring for every 100 mL water. So
a 1,000ml beaker or flask would have 50 drops. Set these up ahead of
time. For purple do half red and half blue drops, for green I would
just use the green food coloring at 5/100mL.
Blood Type A is Red (*or Blue)
Blood Type B is Blue (*or Yellow)
Blood Type AB is Purple (*or Green)
Blood Type O is Clear
Background Information
The following information is from the Red Cross
Almost 40% of the population has O+ blood
Patients with Type O blood must receive Type O blood
About half of all blood ordered by hospitals in our area is Type O
Type O blood is the universal blood type and is the only blood type that can be transfused to
patients with other blood types
Only about 7% of all people have Type O negative blood
Type O negative blood is the preferred type for accident victims and babies needing
exchange transfusions
There is always a need for Type O donors because their blood may be transfused to a person
of any blood type in an emergency
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ABO Blood Type Game

The following lesson is adapted from the Red Cross ABO Blood Type Game Materials 8 3.5 oz cups labeled “ A” 8 3.5 oz cups labeled “ B” 8 3.5 oz cups labeled “ AB” 8 3.5 oz cups labeled “ O” 32 pipettes 1 250 ml bottle water plus 15 drops red food coloring, labeled “A” 1 250 ml bottle water plus 10 drops blue food coloring, labeled “B” 1 250 ml bottle water plus 7 drops red + 5 drops blue food coloring, labeled “AB” 1 250 ml bottle water, labeled “O” 15 12 - well plates 30 worksheets Color Concentration for food coloring: I use the ratio of 5 drops of food coloring for every 100 mL water. So a 1,000ml beaker or flask would have 50 drops. Set these up ahead of time. For purple do half red and half blue drops, for green I would just use the green food coloring at 5/100mL. Blood Type A is Red (or Blue) Blood Type B is Blue (or Yellow) Blood Type AB is Purple (*or Green) Blood Type O is Clear

Background Information

The following information is from the Red Cross

  • Almost 40% of the population has O+ blood
  • Patients with Type O blood must receive Type O blood
  • About half of all blood ordered by hospitals in our area is Type O
  • Type O blood is the universal blood type and is the only blood type that can be transfused to patients with other blood types
  • Only about 7% of all people have Type O negative blood
  • Type O negative blood is the preferred type for accident victims and babies needing exchange transfusions
  • There is always a need for Type O donors because their blood may be transfused to a person of any blood type in an emergency

If your blood type is: Type You Can Give Blood To You Can Receive Blood From A+ A+ AB+ A+ A- O+ O- O+ O+ A+ B+ AB+ O+ O- B+ B+ AB+ B+ B- O+ O- AB+ AB+ Everyone A- A+ A- AB+ AB- A- O- O- Everyone O- B- B+ B- AB+ AB- B- O- AB- AB+ AB- AB- A- B- O- Out of 100 donors..... 84 donors are RH+ 16 donors are RH- 38 are O+ 7 are O- 34 are A+ 6 are A- 9 are B+ 2 are B- 3 are AB+ 1 is AB-

Experiment.

Tell students that:

  • If the color of the “blood” changes, it is not compatible.
  • If the “blood” color stays the same, then it is compatible. A. Patient #1 is Type A. Patient #1 needs a transfusion. Ask students what blood types can this patient receive? For patient #1, tell students to:
  1. Pipette 3 squirts of liquid from Type A into the 1st^ well.
  2. Do a “transfusion” by adding 3 more squirts of Type A to the 1 st^ well and note if there is any change in color. (There is no change.) Explain that No change = Safe, Change = unsafe.
  3. Now pipette another 3 squirts of type A from patient #1 into the second well on row 1
  4. This time, add 3 squirts of Type B to the patient for the ”tranfusion.” a. Students will notice a color change and see the change means that this is Unsafe , b.
  5. Add 3 squirts of Type A to wells 3 and 4 on Row 1 and add Type AB and O to determine if these blood types are safe for patient 1.

Blood Type Chart:

Conclusions Blood type A can only be given to type ____________________patients. Blood type A patients can only receive_____________________type blood. Blood type B can only be given to type ____________________patients. Blood type B patients can only receive_____________________type blood Blood type AB can only be given to type ___________________patients. Blood type AB patients can only receive____________________type blood Blood type O can only be given to type ____________________patients. Blood type O patients can only receive_____________________type blood

Color change

(yes/no)?

A added

Color change

(yes/no)?

B added

Color change

(yes/no)?

AB added

Color change

(yes/no)?

O added

Patient

Type A

Patient

Type B

Patient

Type AB

Patient

Type O