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Amoeba Sisters Enzymes Practice Worksheet, Exercises of Biology

Cambridge CollegeBiology

Typology: Exercises

2020/2021

Uploaded on 04/20/2021

ekayavan
ekayavan 🇺🇸

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AMOEBA SISTERS: VIDEO RECAP
ENZYMES
Amoeba Sisters Video Recap: Enzymes
1. In the box below, please illustrate an enzyme and
substrate. Label the following key words in your
illustration: enzyme, substrate, and active site.
2. Enzymes are typically which type of biomolecule?
_________________________________________________
3. Describe the effects that enzymes can have on substrates.
_________________________________________________
_________________________________________________
_________________________________________________
_________________________________________________
_________________________________________________
In order to function efficiently, enzymes need to be at an ideal pH and temperature.
Different enzymes have different ideal pH and temperature conditions. If the pH or
temperature is extreme for a particular enzyme, it can even denature an enzyme,
which can prevent it from binding and acting on its substrate. For the following two
scenarios, name the variable (temperature or pH) that is affecting the function of the
enzyme.
A) ATP is produced by cellular respiration in your human body cells. There are a variety of enzymes that work to produce
ATP, but one of those enzymes is called phosphofructokinase-1. This enzyme is sensitive to blood acidity. Blood can
become more acidic if a patient is in respiratory distress.
4. Variable affecting enzyme function: ___________________________________________________
B) A popular lab that can be performed by students
is to test the reaction rate of catalase enzyme when
it acts on the substrate hydrogen peroxide. Catalase
has the ability to break down hydrogen peroxide.
Catalase can be found in beef liver from the grocery
store! However, if the beef liver is boiled first, the
catalase will not be able to break down hydrogen
peroxide.
5. Variable affecting enzyme function: ___________________________________________________
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AMOEBA SISTERS: VIDEO RECAP ENZYMES

Amoeba Sisters Video Recap: Enzymes

  1. In the box below, please illustrate an enzyme and substrate. Label the following key words in your illustration: enzyme , substrate , and active site.
    1. Enzymes are typically which type of biomolecule?
    1. Describe the effects that enzymes can have on substrates.

In order to function efficiently, enzymes need to be at an ideal pH and temperature. Different enzymes have different ideal pH and temperature conditions. If the pH or temperature is extreme for a particular enzyme, it can even denature an enzyme, which can prevent it from binding and acting on its substrate. For the following two scenarios, name the variable (temperature or pH) that is affecting the function of the enzyme. A) ATP is produced by cellular respiration in your human body cells. There are a variety of enzymes that work to produce ATP, but one of those enzymes is called phosphofructokinase-1. This enzyme is sensitive to blood acidity. Blood can become more acidic if a patient is in respiratory distress.

  1. Variable affecting enzyme function: ___________________________________________________ B) A popular lab that can be performed by students is to test the reaction rate of catalase enzyme when it acts on the substrate hydrogen peroxide. Catalase has the ability to break down hydrogen peroxide. Catalase can be found in beef liver from the grocery store! However, if the beef liver is boiled first, the catalase will not be able to break down hydrogen peroxide.
  2. Variable affecting enzyme function: ___________________________________________________

AMOEBA SISTERS: VIDEO RECAP ENZYMES

Real Life Enzyme Scenarios

Please fill in the chart for every real life scenario listed below. Some boxes have been filled in for you!

Scenario

Identify

Enzyme:

Identify

Substrate:

Illustrate the

Scenario (label

enzyme and

substrate in

illustration):

Describe the relationship

between the substrate

and enzyme in the

scenario.

Lactase is an enzyme that breaks down a sugar found in dairy products known as lactose. Some people are lactose intolerant, and this can be due to not having enough lactase production. People who are lactose intolerant may not feel well after eating foods containing lactose.

  1. lactose 7. 8. An enzyme called glucocerebrosidase breaks down a glycolipid in the body known as glucocerebroside. However, in a genetic disease known as Gaucher’s disease, the body does not produce enough glucocerebrosidase. Therefore glucocerebroside can build up in the body and this can cause serious side effects such as anemia and swelling of the liver and spleen.

Pancreatitis is an inflammation of the pancreas which can damage pancreatic tissue. The pancreas produces digestive enzymes such as amylase and lipase. These enzymes assist in breaking down certain food biomolecules. In this disorder, enzyme production from pancreatic tissue may be stopped.

      1. Since the pancreatic tissue can be damaged in this disorder, the production of the enzymes in this tissue (amylase and lipase) may be disrupted as well. This would affect the ability to break down certain types of food biomolecules (substrate).