Amoeba sisters video worksheet, Cheat Sheet of Environmental science

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2021/2022

Uploaded on 10/16/2023

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Amoeba Sisters Video Recap: Microscopes
1. Explain why both magnification
and resolution are important in order
to see a microscopic organism.
_______________________________
_______________________________
_______________________________
_______________________________
_______________________________
2. How are electron microscopes
different from light microscopes and
how is their ability to observe a
specimen different?
_______________________________
_______________________________
_______________________________
_______________________________
_______________________________
3. What are two different types of
electron microscopes and how might
their ability to observe a specimen be
different?
_______________________________
_______________________________
_______________________________
_______________________________
_______________________________
4. Label the below objective lenses.
Assuming a 10X eyepiece (ocular
lens), can you give the total
magnification when using the above
objective lenses?
Scanning Objective x Eyepiece
_______________________________
Low Power Objective x Eyepiece:
_______________________________
High Power Objective x Eyepiece:
_______________________________
For the following tip, explain the reasoning for why it should be followed.
It’s important to be aware that many slides and coverslips are made of glass.
5. Reasoning:
For the following tip, explain the reasoning for why it should be followed.
When using a light microscope, focus the specimen with the scanning objective
lens first.
6. Reasoning:
For the following tip, explain the reasoning for why it should be followed.
As you move up to the high power objective lens, avoid using the coarse focus
knob.
7. Reasoning:
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Amoeba Sisters Video Recap: Microscopes

  1. Explain why both magnification and resolution are important in order to see a microscopic organism.





2. How are **electron microscopes** different from **light microscopes** and how is their ability to observe a specimen different? _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ 3. What are two different types of **electron microscopes** and how might their ability to observe a specimen be different? _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ 
  1. Label the below objective lenses. Assuming a 10X eyepiece (ocular lens), can you give the total magnification when using the above objective lenses? Scanning Objective x Eyepiece

Low Power Objective x Eyepiece:


High Power Objective x Eyepiece:


_For the following tip, explain the reasoning for why it should be followed._ **It’s important to be aware that many slides and coverslips are made of glass.** 5. Reasoning: _For the following tip, explain the reasoning for why it should be followed._ **When using a light microscope, focus the specimen with the scanning objective** **lens first.** 6. Reasoning: _For the following tip, explain the reasoning for why it should be followed._ **As you move up to the high power objective lens, avoid using the coarse focus** **knob.** 7. Reasoning: 

Amoeba Sisters Video Recap: Microscopes

  1. Explain, in your own words, how you would use the light microscope to view a wet mount of a protist, assuming the microscope is plugged in and that the light source is on. Please use at least the following 9 structures in your description and underline them as you use them: stage , stage clip(s) , stage knob(s), eyepiece , scanning objective lens , low power objective lens , high power objective lens , coarse focus knob , and fine focus knob.








  1. For the below light microscope, label all structures you were asked to include in the previous question (9 structures) and additionally label the condenser , diaphragm , and light source. Check that you have 12 structure names labeled on the below microscope. True and False Quick Check Place “T” for true and “F” for false.
  2. ____ Follow the procedure for a wet mount to avoid air bubbles. An air bubble may be mistaken for the specimen.
    1. ____ The pointer that you see in view can typically be rotated by rotating the eyepiece (ocular lens).
    2. ____ If the lens needs to be cleaned , gently use tissue paper to wipe the lens thoroughly.
    3. ____The light microscope drawn is also known as a compound microscope as it has more than one set of lenses.
    4. ____ Oil immersion can be used at all magnification levels to increase resolution.