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Using an ASSET Microscope: A Comprehensive Guide for Beginners, Study notes of Anatomy

The University of SheffieldAnatomy

A step-by-step guide on how to use an asset microscope, including preparing the specimen, adjusting the focus and light, and handling the microscope safely. It also mentions the asset program at cornell university funded by nih sepa.

Typology: Study notes

2021/2022

Uploaded on 09/12/2022

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ASSET MICRO 1 Microscope Anatomy
Eyepiece (10x)
Arm
Objectives
(4x, 10x, 40x)
Stage
Base
Course Adjustment Knob
(Use on Lower Power)
Fine Adjustment Knob
(Use on Higher Power)
Stage Clip
Fluorescent Light
Diaphragm
Condenser
Stage
Adjustment
The ASSET Program (Advancing Secondary Science Education through Tetrahymena) at Cornell University is funded by NIH SEPA
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Download Using an ASSET Microscope: A Comprehensive Guide for Beginners and more Study notes Anatomy in PDF only on Docsity!

ASSET MICRO 1 Microscope Anatomy

Eyepiece (10x)

Arm

Objectives

(4x, 10x, 40x)

Stage

Base

Course Adjustment Knob

(Use on Lower Power)

Fine Adjustment Knob

(Use on Higher Power)

Stage Clip

Fluorescent Light

Diaphragm

Condenser

Stage

Adjustment

How to Use ASSET Micro I Microscopes

  1. Holding the microscope by the Arm and the Base (always use both hands for safety), move the microscope to the location in the room where you have access to a power outlet.
    1. Plug in the microscope and turn on the light using the green light switch on the back of the base. The light may flicker initially as the fluorescent bulb is warming up.
  2. Take note of how strong your microscope’s objectives are. Remember to multiply the power of the eyepiece by the power of the objective you are using. The eyepiece is 10x (10 times the power of the human eye) and the objects are 4x, 10x and 40x. Your lowest magnification is (10x)(4x)= 40x. Your highest magnification is (10x)(40x)= 400x.
    1. Before turning on your microscope, do a general look-over and make sure you know the function of all the parts of the microscope. Use the Microscope Anatomy Guide to help with this process. Be gentle when turning knobs and moving levers. any knobs and always be extremely careful when using microscopes. Each microscope has subtle differences, so if you have used a microscope before some things maybe different. X
  3. Prepare your specimen using a microscope slide. If you will be using the highest power objective, you will need to use a coverslip so you don’t get sample onto the objective lens. We also recommend lowering the cover slip from a 45 degree angle to minimize air bubbles.
  4. Once your slide is ready, place it on the stage. Make sure your objective is on the lowest power to start. It is easiest to find your specimen on the lower power before using the higher powers.

How to Use ASSET Micro I Microscopes... continued

  1. Now, click over to the highest power, 40x. Again you may need to move the stage to center your image. Also, now is when you want to use the fine adjustment to sharpen the image. Adjust the focus slowly. Again . If you move too quickly you could move the slide or damage the lens. If you have problems, switch back down to 4x and start the process again.
  2. If at any point you lose the specimen from your line of vision, switch down to the lower powers and follow the steps again. Once you are in the highest power, it is harder to find what you are looking because you’re only looking at a tiny part of the sample.
  3. The light will likely need to be adjusted at the higher power to allow you to see contrasts through the eyepiece. Use the silver diaphragm lever to adjust your light quantity.
  4. Once you are finished with a slide, click over to the lowest objective (4x) and lower the stage away from the objectives using the course adjustement knob. Now it is safe to remove the slide from the microscope.
  5. If you are continuing to use the microscope you can either prepare a new slide or clean your current slide before going on. Folllow the same steps to examine your next specimen.
  6. When you are completely done using your microscope, make sure that the 4x objective is in position and make sure all slides have been re- moved. Unplug your microscope and wrap the cord around the base. Please cover the microscope with the provided microscope bag to minimize dust exposure. Carrying the microscope by the arm and the base, return it to its storage area.
  7. Return to your work station and make sure all slides are cleaned and put away and your work area is spotless! Working with microscopes has a steep learning curve! With each use your skills will improve quickly!