BIOL 1107L Week 10 PCR & Bioinformatics
Assignment: Molecular Applications Post Lab
After running the BLAST search on the sequence of a sample from Patient #347. Complete the following
questions based on the search results. (10 pts total)
1. Record the Accession of the top hit (0.5 pt):
QJD23475.1
2. Based on the E-value how confident can you be in this being a biologically relevant alignment?
Explain your reasoning. (1 pt)
The e-value is 0.0 and the e value less than 0.03 means its perfectly matched so we are very confident this can be a
biologically relevant alignment.
3. Based on the description of the top hit what is the possible diagnosis of Patient #347? (1 pt)
Coronavirus is the possible diagnosis of Patient #347
Click on the Accession for the top hit and answer the questions below:
4. What is the specified source for this match? (0.5 pt)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
5. What was the technology used for obtaining the sequence of the match? (0.5 pt)
Sequencing technology: : Illumina
6. Based on the information provided about the sequence matched, what possible geographical region
did this patient visit recently? (1 pt)
Country: USA: NY
2020-03-18
Return back to the search results, select the second Accession # and answer the questions below.
7. What is the E-value for this Accession? (0.5 pt)
0.0
8. What is the value of the Percent Identity and what does it indicate? (1.5 pts)
99.67 indicates that this result is a 99.67% match to test subjects.
9. Identify the source of this match: (0.5 pt)
