Download BIO60204 PRAC 1 FLOW CHART and more Schemes and Mind Maps Biochemistry in PDF only on Docsity!
Part 1: Effect of concentration on pH Add 10 mL of 0.10 M phosphate buffer pH 7. Add 10 mL of 0.10 M sodium acetate buffer Record initial pH Add 10 mL of 0.10 M Tris buffer pH 8. Make a final concentration of 0.01 M Make a final concentration of 0.01 M Make a final concentration of 0.01 M Add distilled water Add distilled water Add^ distilled^ water Step 1 Step 2 Take 10 mL of the solution Take 10 mL of the solution Take 10 mL of the solution Make a final concentration of 0.0 0 1 M Make a final concentration of 0.0 0 1 M Make a final concentration of 0.0 0 1 M Step 3 Step 4
- Measure the pHs of the undiluted and diluted solutions
- Add 10 drops of NaOH
- Measure the pH again
Part 2: Why a buffer is a buffer? Step 2a Put 20 mL of 0.10 M Tris buffer pH 8 .0 [pKa=8.14] in a beaker Add 1 mL of 0.5 M NaOH to it Mix well using a glass rod and record the pH Step 2 b Put 20 mL of 0.1 M Sodium acetate buffer in a beaker Add 1 mL of 0.5 M HCl to it Mix well using a glass rod and record the pH Step 2 c (1) Put 20 mL of distilled water in a beaker (2) Add 1 mL of 0. M NaOH to it (3) Mix well using a glass rod and record the pH Repeat Step 2c. Add 1 mL of 0.5 M HCl to it Mix well using a glass rod and record the pH
- Incubate the buffers for 15 minutes
- Record their initial pH of each solution
- Add 1 mL of 0.2 M NaOH to this sample
- Mix well using a glass rod and record the pH
