Biochemical Techniques: Study Questions
1. What is a cell homogenate?
2. Why is uniformity important when preparing a homogenate?
3. What is the difference between a pellet and a supernatant?
4. How does differential centrifugation separate cell components?
5. How does density gradient centrifugation differ from differential
centrifugation?
6. What is a sedimentation coefficient?
7. What is the difference between salting in and salting out?
8. What is the purpose of dialysis?
9. What are the stationary and mobile phases in column
chromatography?
10. How does size exclusion chromatography separate proteins?
11. How does ion-exchange chromatography separate proteins?
12. What is the difference between cation exchange and anion exchange
chromatography?
