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BIOS 242 Week 8 Assignment; Lab Practicum, Papers of Biology

Chamberlain College of NursingBiology

BIOS 242 Week 8 Assignment; Lab Practicum

Typology: Papers

2023/2024

Available from 09/04/2024

Guide4Students
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STUDY LAB REPORTS
 Slant Agar Tube
 Growing at room temp: Pink Color
Isolation (Streak Transfer)
 Inoculation
 Incubation (37 celsius)
 Identify Streak Plate
 Know Parts of microscope
 40x lens= 400 magnification (40 x 10)
 Naseria gonnerah (gram negative, pink)
 E. coli (rods)
Plasmodium (little purple things inside blood cells)
 Trapanosomes (outside blood cells)
 B. Sub (Strepto bacilli)
 Strepto coccus (mardigras beads)
 Know spiral bacteria
 Simple staining: looking at different staining, one stain
Inoculation, let it dry, heat fix. If you don’t let it dry it will boil and explode/lose
their shape
Adding dye, if you don’t heat fix before dye will wash off
 Safromyces cervisiea (yeast cells): big and overly round
 Cheek Cells (rectangular, clear nucleus)
 S. Epi (Staphococulus staph o stack)
 Differential Staining (gram staining)
Gram positive: purple
Gram negative: pink
 At the beginning, put bacteria on the slide, let it dry, heat fix, do staining.
 First dye: Chrystal violet gram- positive will be purple, gram-negative will be purple
 Second dye: Iodine gram- positive will be purple, gram-negative will be purple
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STUDY LAB REPORTS

 Slant Agar Tube  Growing at room temp: Pink Color Isolation (Streak Transfer)  Inoculation  Incubation (37 celsius)  Identify Streak Plate  Know Parts of microscope  40x lens= 400 magnification (40 x 10)  Naseria gonnerah (gram negative, pink)  E. coli (rods) Plasmodium (little purple things inside blood cells)  Trapanosomes (outside blood cells)  B. Sub (Strepto bacilli)  Strepto coccus (mardigras beads)  Know spiral bacteria  Simple staining: looking at different staining, one stain  Inoculation, let it dry, heat fix. If you don’t let it dry it will boil and explode/lose their shape  Adding dye, if you don’t heat fix before dye will wash off  Safromyces cervisiea (yeast cells): big and overly round  Cheek Cells (rectangular, clear nucleus)  S. Epi (Staphococulus staph o stack)  Differential Staining (gram staining)  Gram positive: purple  Gram negative: pink  At the beginning, put bacteria on the slide, let it dry, heat fix, do staining.  First dye: Chrystal violet gram- positive will be purple, gram-negative will be purple  Second dye: Iodine gram- positive will be purple, gram-negative will be purple

 Third step: Ethanol- 15 seconds gram-positive will be purple gram-negative will be clear  Final step: Saffarin gram-positive will be purple, gram-negative will be pink  If alcohol is left too long, they will all be pink  If you use bad alcohol, everything will be purple  Alcohol is the main differential step  Carbohydrate fermentation: Different bacteria can ferment different sugar (lactose, dextrose)  Fermentation two products: Acid and gas (yellow: fast fermenter, orange: slow fermenter) Antibiotics  Penicillin inhibits cell wall Clindamycin inhibits protein synthesis  Polymyxin inhibits cell membrane Trimethoprim: Fluoroquinolones: DNA  Rifampin: RNA  Tetracycline: Protein  Vancomycin: Cell Wall  Erythromycin: Protein  Streptomycin: Protein  Ampicillin: Cell Wall  Cell Wall: Beta Lactams  Disinfectants (adding disinfectant at 0 min, 1 min, 5 min, 10 min)  Kirby Bauer (Adding antibiotic discs)  Lots of emptiness: Bacteria is susceptible to antibiotic  Growth all the way up to the disk: antibiotic-resistant  Fomites  Food Safety How germs spread  Skin bacteria  Pink plate, allow staph to grow but not e. coli: MSA PLATE  Pink plate, e. coli growing but staph and strep aren’t growing: MAC PLATE  Gram-positive doesn’t grow