Co-crystal Preparation: Strategies and Techniques for Pharmaceutical Applications, Slides of Biology

Download Co-crystal Preparation: Strategies and Techniques for Pharmaceutical Applications and more Slides Biology in PDF only on Docsity!

co-crystal preparation

Co-crystals, which retain the unique crystalline structures with their multiple components,

have become a very important subject of study in the field of pharmaceuticals (i.e., drug

discovery & design). Creative Biostructure provides high-quality services to streamline the

procedures of protein preparation, co-crystal generation and structure determination with the

UniCrys™ X-ray crystallization platform.

For co-crystal preparation, strategies like co-crystallization and soaking are employed with all important variables carefully tested: 1) methods for ligand addition into the protein sample; 2) incubation period; 3) effects of additive components, etc. As the conditions for binding assays and crystallization trials vary, extensive protocol optimization will be carried out for obtaining diffraction-quality crystals. Co-crystal generation methods

• Co-expression
• Co-purification
• Co-crystallization
• Soaking

High-quality crystals and synchrotron light sources produce high- resolution co-crystal structures, which provide high-value insights into the binding mechanism and facilitate the drug design process. At Creative Biostructure, a variety of techniques are used for the accurate structure determination with refined electron density maps of the ligand molecules plotted (see Protein Crystallization and Structure Determination).