Epigenetic Modifications &
Epigenomic Technologies
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Epigenetic Modifications & Epigenomic Technologies, Slides of Biotechnology
Epigenetics can be used to explain the phenomena that cannot be explained by genetics/genomics, such as the differences between monozygotic twins, which are considered to be genetically identical.
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2019/2020
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Epigenetic Modifications &
Epigenomic Technologies
CONTENTS
EPIGENETICS BASICS DNA METHYLATION HISTONE MODIFICATION
- (^) Definition
- (^) Modifications
- (^) Introduction
- (^) Function
- (^) Introduction
- (^) Research Methods
DNA
methylation Histone Modification Histone Modification Epigenetic modifications Chromatin accessibility transcription patterns
Types of Epigenetic Modifications
Functions of Epigenetic Modifications
2 DNA METHYLATION
Where does DNA methylation occur? What are the effects of DNA methylation?
- (^) Repression of transposable elements or highly transcribed genes
- (^) Cell differentiation and embryonic development
- (^) Association with silencing histone modifications
- (^) Vertebrates: almost exclusively within CpG islands
- (^) Plants: CpG and nonCpG (primarily CpNpG and CpHpH, where H = A, T, C)
MeDIP-Seq
Workflow of MeDIP-Seq
Fragmentation Ligation Denaturation
Y
Immunoprecipitation Purification Amplification Sequencing Sequencing Cancer Normal
Histone modification types:
Histone modifications may affect chromatin accessibility and gene expression. Example: H3K4me3 is a mark associated with transcriptionally active chromatin; H3K27me3 leads to compact chromatin, which represses gene expression.