Download Exam 1 | BLD 430 - Molecular Lab Diagnostics and more Quizzes Biology in PDF only on Docsity!
Log of the Odds (LoD)
Score
Used to calculate how likely genes are to be linked 3 is the magic number If close to 3 run it again using more people TERM 2
Hardy-Weinberg equilibrium
DEFINITION 2 p^2 + 2pq + q^2 = Assumptions: No selection advantage Large Population Random mating Assumptions do not hold true most of the time. TERM 3
Multigenic
DEFINITION 3 Result of more than one gene Eye color Some diseases TERM 4
Epistasis
DEFINITION 4 Expression of a gene depends on the presence of one or more "modifier genes." TERM 5
Temperature of Melting (DNA)
DEFINITION 5 2(AT) + 4(CG) = degrees CelsiusExampleATCGAAATTTCG2(8)
Nucleic Acid Isolation
Bind it to silica or glass (Guanidium thicynate) Magnetic (high throughput) Contaminants:RNAases (be carfeul if separating RNA)ProteinsLipidsCarbohydratesPhenol (if organic extraction) TERM 7
How to calculate Purity and weight
DEFINITION 7 260 / 280 = OD260/2802.0 = 100% pureCan usually work with 1.8 - 2.0Run gel electrophoresis to determine if DNA is HMW. TERM 8
Concentration of DNA calculation (ug/uL)
DEFINITION 8 OD260 x 50 x dil. factor / 1000 ul/ml = ugs/ml TERM 9
Storage of DNA
DEFINITION 9 4C (short term) or frozen in 100 mM tris or tris EDTA -80C for RNA TERM 10
Ligase
DEFINITION 10 Joins two strands of DNA by repairing nicks in the phosphate backbone
Kinase
Adds a phsphoryl group Use to label reactions TERM 17
Terminal Transferase
DEFINITION 17 Adds bases at the 3' end of molecules TERM 18
Cleavase
DEFINITION 18 Cuts unboudn "flaps" of DNA in artificial structures
