Study with the several resources on Docsity
Earn points by helping other students or get them with a premium plan
Prepare for your exams
Study with the several resources on Docsity
Earn points to download
Earn points by helping other students or get them with a premium plan
Class: BLD 430 - Molecular Lab Diagnostics; Subject: Biomedical Laboratory Diagnost; University: Michigan State University; Term: Spring 2013;
Typology: Quizzes
1 / 13
This page cannot be seen from the preview
Don't miss anything!
short, single-stranded DNA or RNA molecules TERM 2
DEFINITION 2 10-20 ugs/mL TERM 3
DEFINITION 3 There is no DNA in a Red Blood Cell TERM 4
DEFINITION 4 Hunk, must be quickly processed TERM 5
DEFINITION 5 Preserved[[Image:Frozen tissue array block.jpg|300px|thumb|right|A Frozen Tissue Array Block]]
For staining, heat fixed, cells are killed TERM 7
DEFINITION 7 2(AT)+4(CG) good for short sequences, approximate TERM 8
DEFINITION 8
DEFINITION 9 SDS- sodium dodecyl sulfatedisrupts membrane TERM 10
DEFINITION 10 Proeinase K chews up protein
proteins lipidscarbsphenol TERM 17
DEFINITION 17 Good- 1.4-2260/ TERM 18
DEFINITION 18 an enzyme that can catalyze the joining of two large molecules by forming a new chemical bondJOINS! between oligonucleotides TERM 19
DEFINITION 19 an enzyme whose central biological function is the synthesis of polymers of nucleic acids.elongates, reads and fills in the rest of the bases TERM 20
DEFINITION 20 Reverse transcriptase is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.runs opposite direction
A restriction enzyme is an enzyme that cuts DNA at specific recognition nucleotide sequences known as restriction sites.generates fragmentscuts dsDNA produces sticky/blunt ends TERM 22
DEFINITION 22 Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end of a polynucleotide chain.removes any ds barriers TERM 23
DEFINITION 23 removes a phosphate group TERM 24
DEFINITION 24 adds a phosphoryl group TERM 25
DEFINITION 25 adds bases to the 3' end of molecules
Castloadrun/ electrophoresis - supply currentStain document TERM 32
DEFINITION 32 TAE TBE TERM 33
DEFINITION 33 Ethidium BromideSybrGreenSilverstain TERM 34
DEFINITION 34 goes inbetween bases, interacts with but also binds TERM 35
DEFINITION 35 binds to ds DNA doesnt interchelate
proteins lower level of detection (picks up on very small amout) TERM 37
DEFINITION 37 Pulsed field gel electrophoresis is a technique used for the separation of large DNA molecules by applying an electric field that periodically changes direction to a gel matrix. TERM 38
DEFINITION 38 conformational difference of single-stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions TERM 39
DEFINITION 39 A heteroduplex is a double-stranded molecule of nucleic acid originated through the genetic recombination of single complementary strands derived from different sources, such as from different homologous chromosomes or even from different organisms. TERM 40
DEFINITION 40 In molecular biology, restriction fragment length polymorphism, or RFLP, is a technique that exploits variations in homologous DNA sequences.
In situ hybridization is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ), or, if the tissue is small enough (e.g. TERM 47
DEFINITION 47 without gel- in a tubeuse fluorescent label- probe, binging dye TERM 48
DEFINITION 48 has to cross this threshold at or before a certain number of cycles for it to be positive TERM 49
DEFINITION 49 enzymes cleave after specific base or base combos ---never used TERM 50
DEFINITION 50 Sanger gilbert used ddNTP's dont have 3' hydroxyl doesn't allow extension
Sequence from the bottom upsmallest fragments are at the bottom TERM 52
DEFINITION 52 Everytime you ass a base, there is a phosphate that is lost and then captured to generate light where the light is measured TERM 53
DEFINITION 53 specimen and ID integrity throughout the processqualification and responsibilities quality assurance plan proficiency testing TERM 54
DEFINITION 54 lab director- MD, PhD = they are ultimately responsible technical supervisor- make sure assays run right and trainClinical consultant- MD, PhD =communicate what results mean, what to runGeneral supervisor- technologist TERM 55
DEFINITION 55 preanalytic- prep, right test orderedanalytic- testing processpostanalytic- reporting results
high risk, requires pre-market approval *most molecular tests TERM 62
DEFINITION 62 1- general purpose 2-RUO- research use only3- IUO- investigational use only4- kits (class II and III)5- immunohistochemicals (I, II, III) 6- ASR- analyte specific reagents
