Exosome Characterization, Slides of Biology

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Exosome Characterization

Creative Biostructure’s exosome analysis and characterization service makes it easy to profile exosomal lipids,

proteins and RNAs with our years of experience working on exosomes. Our expertise provides comprehensive

support for your exosome engineering study by including the following customized services.

The Importance of Exosome Analysis and Characterization

Characterization of exosomes has become a topic of interest in medical research and molecular biology

because it can provide very valuable information for early detection, disease monitoring and development of

effective treatments against cancer and autoimmune diseases. Exosomes are known as one type of secreted

membrane vesicles that are responsible for intercellular communication by providing means for intercellular

exchange of proteins, lipids, mRNA, miRNA and DNA, contributing to intercellular communication in relevant

biological processes, playing an important role in the development of several diseases, and specifically,

modulating cancer microenvironment and the immune response. Exosomes and its contents also have

emerged as non invasive biomarkers for various diseases. Exosomes composition and quantity may be‐invasive biomarkers for various diseases. Exosomes composition and quantity may be

directly related to the pathological state of the patient, so the characterization of exosome could be of great

relevance for diagnosis.

Therefore, to acquire a deeper knowledge of the exosome biology and accelerate the use of these

extracellular vesicles in diagnostic tests or clinical research, Creative Biostructure provides a sensitive,

reproducible methods for exosome characterization.

Exosomal Lipidomics & Metabolomics Services

Lipids, an important part of cellular physiology, are the major components of exosomes. The

importance of lipids in exosome biology has been associated with the fact that lipid content of

exosomes differs from the parent cells and also changes during physiological processes. Exosome

lipidomics study provides a great access to identify lipid-based biomarkers and unravel its

monitoring function in diseases. Besides, a wide range of metabolites in exosomes could also be

used for biomarker discovery by showing distinct metabolite populations during different disease

treatment stages.

Our customized services provide comprehensive support for exosomal lipidomics & metabolomics

study by using different analysis methods for data acquisition, analysis, and even interpretation:

Thin layer chromatography (TLC), High performance liquid chromatography (HPLC), NMR

spectroscopy, Mass spectrometry, etc.

Exosomal Proteomics Services

Like exosomal lipidomics, exosomal proteomics also have been recognized an easy access for

biomarkers monitoring, providing information on the physiology study of the parent cells and

exosomes. For analysis by mass spectrometry, exosomal proteins can be separated by SDS-PAGE,

in gel digested, followed by LC-MS/MS experiments. Based on our customer need, we can work

from exosome isolation to data collection and analysis, a final report with results will be provided.

Exosomal RNA Sequencing Service

Nucleic acid is another important cargo that exosomes carry, including deoxynucleic acids (DNA), coding and

non-coding ribonucleic acid (RNA) like messenger RNA (mRNA) and microRNA (miRNA). After exosome

isolation, nucleic acids need to be accurately quantified in order to track many kinds of medical conditions and

diseases, especially when nucleic acids of exosomes are greatly different from the parental cell content.

Creative Biostructure provides a complete exosome workflow solution to solve nucleic acids profile of

exosomes, starting from fast and efficient isolation of RNA to exosomal RNA sequencing using qRT-PCR and

novel sequencing techniques.

Based on the comprehensive techniques in exosome analysis and characterization, Creative Biostructure offers

top services to satisfy your demands on exosome study. Please contact us for more information.

Thank you!

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