Exosome Isolation and Purification: Techniques and Importance, Slides of Biology

Download Exosome Isolation and Purification: Techniques and Importance and more Slides Biology in PDF only on Docsity!

Exosome

Purification

The importance of exosomes in the pathology of key diseases have made them popular targets in both basic and pharmaceutical research, therefore, isolation of exosome becomes a prerequisite step before furthering their applications. Creative Biostructure provide different techniques to isolate exosomes from various sample matrices.

Exosome Isolation Techniques In order to isolate exosomes reliably and efficiently from complex biological matrices like blood, urine, and body fluid, Creative Biostructure has developed five groups of exosome isolation techniques: ultracentrifugation-based techniques, size-based techniques, immunoaffinity capture-based techniques, exosome precipitation, and microfluidics-based techniques. Creative Biostructure provides reliable and reproducible services to isolate exosomes from almost any biofluid in different scales.

Size-based Isolation Techniques Size-based exosome isolation techniques is a size dependent method to isolate exosomes by using membrane filters with defined molecular weight or size exclusion limits. Different size-based isolation methods could be applied based on customers’ request. We could provide the following size-based isolation techniques: ultrafiltration, syringe filter- based rapid fractionation, sequential filtration, size exclusion chromatography, flow field-flow fractionation, hydrostatic filtration dialysis.

Immunoaffinity Capture-based Techniques The most accurate tool for applying for exosome purification is immunoaffinity. Taking advantage of the presence of plenty of proteins and receptors in the membrane of exosomes, immunoaffinity capture-based technique has been developed for the isolation of exosomes by tapping on immunoaffinitive interactions between those proteins (antigens) and their antibodies, and interactions between the receptors and ligands. A microplate-based enzyme-linked immunosorbent assay (ELISA) has been developed for capturing and quantifying exosomes from plasma, serum, and urine. A combination of other isolation techniques with immunoaffinity capture-

Microfluidics-based Isolation Techniques To further provide customized service to meet our customer needs, Creative Biostructure offers advanced and specialized isolation techniques by tapping on both the physical and biochemical properties of exosomes. In addition to the usual approaches mentioned above, innovative sorting mechanisms such as acoustic, electrophoretic and electromagnetic manipulations can be implemented to broaden our capability on exosome isolation with significant reduction in sample volume, reagent consumption, and isolation time.

Thank you

Excerpt from:^!

https://www.creative-biostructure.com/exosome- isolation-and-purification-650.htm