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FAB classifications
MPO - correct answer Myeloperoxidase: Present in granules of granulocytes Neutrophils:+ Monocyte: w+ Lymphocyte: - Differentiates myeloid from lymphoid SBB - correct answer Sudan black: pospholipids present in immature cells Neutrophil: + Monocyte: + Lymphocyte: w+ Differentiates immature cells NSE - correct answer Nonspecific esterase: alpha-naphthyl butyrate alpha-naphthyl acetate Neutrophil: - Monocyte: + with butyrate Lymphocyte: - Megakaryocyte: + with acetate Differentiates between monocytic and megakaryocyte cells
SE - correct answer Specific esterase: Chloroacetate esterase Neutrophil: + Monocyte: - Lymphocyte: - Differentiates neutrophils from other cells PAS - correct answer Periodic-acid schiff: glycol groups Erythrocytes + Neutrophil: w+ Monocyte: - Lymphocyte: w+ Looks for erythroblast TDT - correct answer Terminal deoxynucleotidyl transferase: DNA polymerase Indirect immunofluorescence Myeloid: - Lymphoid: + Differentiates lymphoid from myeloid AML-M0 - correct answer Acute myelogenous leukemia minimally differentiated
MPO+++
SBB++
TdT- NSE- PAS- Faggo cells present (auer rods in promyelocyte) Bleeding is common (DIC) AML-M3m - correct answer Acute Promyelocytic leukemia Promyelocytes: hypogranular MPO + SBB w+ TdT- NSE- PAS- AML-M4 - correct answer acute myelomonocytic leukemia Myeloid and monocytic features MPO + SBB + TdT- NSE+
PAS-
AML-M4e - correct answer Acute myelomonocytic leukemia Myelomonocytic: eosinophilia MPO + NSE w+ SE + Cyanide resistant peroxidase + AML-M5a - correct answer Acute Monocytic Leukemia Monocytic: poorly differentiated MPO w+ SBB - TdT - NSE + SE + butyrate PAS - AML-M6 - correct answer Acute erythroid leukemia Erythroleukemia MPO - SBB +
ALL-L1 - correct answer Small cell leukemia: homogeneous Small cells Little cytoplasm (dark blue -basophilic) Rare nucleoli ALL-L2 - correct answer Large cell leukemia: heterozygous Large cells Variable cytoplasm (basophilic) Nucleoli present ALL-L3 - correct answer Burkitt's type leukemia Heterozygous Large cells Moderate cytoplasm (vacuoles) Nucleoli Vacuoles are PAS + Myeloid Flow cytometry - correct answer CD CD
MPO
Monocytic Flow cytometry - correct answer CD Erythroid Flow cytometry - correct answer CD GlyA Megakaryocytic Flow cytometry - correct answer CD CD Lymphoid B-ALL Flow cytometry - correct answer CD CD Kappa Lambda Lymphoid Flow cytometry - correct answer CD CD CD CD
