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MICROBIOLOGY LAB PRACTICAL EXAM 1, Study notes of Biology

Biology

MICROBIOLOGY LAB PRACTICAL EXAM 1

Typology: Study notes

2025/2026

Available from 06/27/2026

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MICROBIOLOGY LAB PRACTICAL EXAM
1
Wetaomountaosteps:ao-aoans--1.waterao
2.aospecimenao
3.aoCoveraoslipaooveraospecimen
hangingaodropaosteps:ao-aoans--1.aoVaselineaoaroundaocoveraoslip
2.aomicroaoorganismaoinaocenteraoofaocoveraoslip
3.aoplaceaoconcaveaoslideaoonaocoveraoslip
Gramaostainaosteps:ao-aoans--1.aoCrystalaoVioletao
ao2.aoGram'saoIodineao
3.aoEthylaoAlcohol
4.aoSafraninaoRed
negativeaostainaosteps:ao-aoans--1.aonigrosinao
2.heataoloop
3.bacteriaao
4.aoslideaotoaospreadaomixture
5.aoairaodry
acid-fastaostainaosteps:ao-aoans--1.aocarbolfuchsin
2.aoacidaoalcoholao
3.aomethyleneaoblue
4.aowashao&aodry
simpleaostainaostepsao-aoans--1.aofixedaosmearaoslide
2.aodyes
3.aorinseao&aodry
nutrientaobrothao-aoans---liquidaomediaaousedaotoaogrowaobacteria
agarao-aoans---usedaotoaocultureaomicroorganisms
candleaojarao-aoans---usedaoforaoanaerobicaoorganisms
Petriaodishao-aoans---Usedaotoaocultureaooraoobserveaobacteriaaogrowth
pf3
pf4
pf5

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MICROBIOLOGY LAB PRACTICAL EXAM

Wetaomountaosteps:ao-aoans--1.waterao 2.aospecimenao 3.aoCoveraoslipaooveraospecimen hangingaodropaosteps:ao-aoans--1.aoVaselineaoaroundaocoveraoslip 2.aomicroaoorganismaoinaocenteraoofaocoveraoslip 3.aoplaceaoconcaveaoslideaoonaocoveraoslip Gramaostainaosteps:ao-aoans--1.aoCrystalaoVioletao ao2.aoGram'saoIodineao 3.aoEthylaoAlcohol 4.aoSafraninaoRed negativeaostainaosteps:ao-aoans--1.aonigrosinao 2.heataoloop 3.bacteriaao 4.aoslideaotoaospreadaomixture 5.aoairaodry acid-fastaostainaosteps:ao-aoans--1.aocarbolfuchsin 2.aoacidaoalcoholao 3.aomethyleneaoblue 4.aowashao&aodry simpleaostainaostepsao-aoans--1.aofixedaosmearaoslide 2.aodyes 3.aorinseao&aodry nutrientaobrothao-aoans---liquidaomediaaousedaotoaogrowaobacteria agarao-aoans---usedaotoaocultureaomicroorganisms candleaojarao-aoans---usedaoforaoanaerobicaoorganisms Petriaodishao-aoans---Usedaotoaocultureaooraoobserveaobacteriaaogrowth

slantao-aoans---aaomediumaoforaowhichaobacteriaaocanaogrowaoon. E.coliaoGramaostainao-aoans--redaocoloredaorods S.aoepidermidisaoGramaostainao-aoans--purpleaocoloredaograpes B.aosubtilisaoGramaostainao-aoans--purpleaocoloredaorods listaoinformationaogainedaofromaoa particularaoslideaobasedaoonaostainingaotechniquesao(cellaomorphology,aoarrangement,aoc ellaowallaostructure,aoGramaostainaoreactionaooraootheraostainingaoreaction).ao-aoans--... showaomotilityaoonaoaaohangingaodropao-aoans--movementaobecauseaoofaoflagella showaomotilityaoonaoaaoBrownianaomovement.ao-aoans-- bacteriaaomovesaorandomly,aodueaotoaotheaoenergyaoofaoaaosystemao(vibration) whyaodoesaomotilityaooccuraoinaobacteriaao?ao-aoans-- theyaohaveaoaaoflagellumaothataopropelsaothemaoaround. whyaodoesaomotilityaooccuraoinaoprotozoans?ao-aoans--1.aoFlagellaao2.aoCiliaao3.aoAmoeba asepticaotechniqueaostepsao-aoans--1.aoflameaoloop 2.aoflameaotube 3.aoinoculateaobacteria 4.aoflameaoloopao 5.aoflameaotube solidaocultureaosetaoupao-aoans--1-aowater 2-heataoloop 3-bacteria 4-airaodry 5-applyaoheat brothaocultureaosetaoupao-aoans--1.aoheataoloop 2.aobacteriaao 3.aoairaodry 4.aoheataofix 5.aomethanol 6.aoairaodry 7.aoheataofix howaodoaoyouaoproperlyaodryaoandaofixaoaaoslideao?ao-aoans---heataoloop

  • aoaddaobacteriaao -airaodry

phenylalanineaodeaminationaotestao-aoans--- organismsaoproducingaotheaoenzymeaodeaminase

  • aodarkaogreenao(positiveaoresult.) hydrogenaosulfideaoproductionao-aoans--organismsaothataoreduceaosulfur- containingaocompoundsaotoaosulfidesao. blackaoprecipitateaoisaotheaoresult MIOaotestao-aoans---determinesaomotilityaoofaomicroorganisms -ornithineaodecarboxylaseaoactivityaoandaoindoleaoproduction.ao -motilityaoisaoindicatedaobyaocloudinessao
  • aoredaoisaopositiveaoforaoindole. -purpleaoisaopositiveaoforaoornithine oxidaseaotestao-aoans--organismaothataomakeaocytochromeaocaooxidase Positiveaoreactionsaoisaoblue/prurple catalaseaotestao-aoans--enzymeaothataobreaksaodownaoh2O2aotoaoO2aoandaoH2O -positiveao=aobubbling. -negativeao=aonoaobubbles Knowaohowaotoaoloweraotheaooxygenaocontentaosoaothataoanaerobesaocanaogrow.ao- aoans---useaoaaocandleaojar -useaoanaoanaroabicaojar Knowaowhereaobacteriaaowillaogrowaoinaoaaotubeaoofaosolidaomediumaobasedaoonaooxygen aorequirements.ao-aoans---obligateaoaerobes-aogrowaoonaotop -obligateaoanarobes-growsaoonaobottom -microaerophiles-aogrowsaobetweenaobottomaoandaotop what'saotheaopurposeaoofaousingaodilutionaotechniquesaotoaoisolateaobacteria?ao-aoans-- isolatesaobacteria streakaoplateao-aoans---heataoloop -scoopaobrothaoonceaoandaostreakaoquandrantao 1 -heataoloop -streakaoquadrantao 2 -heataoloop -quandrantao 3 -heataoloop -quandrantao 4

streakaoplateaovsaopouraoplateaodilution.ao-aoans--- producesaoindividualaocoloniesaoonaoanaoagaraoplate -determinesaotheaonumberaoofaomicrobesaoinaoaaospecimen Pellicleaonutrientaobrothaotubeao-aoans-- flocculentaonutrientaobrothaotubeao-aoans-- sedimentaonutrientaobrothaotubeao-aoans-- turbidityaonutrientaobrothaotubeao-aoans-- Knowaohowaotoao(listaotheaosteps)aoviewaoaaomicroscopeaoslideaounderaoallaoobjectiveaole nses, includingaohowaotoaouseaotheaooilaoimmersionaoobjective.aoKnowaowhichaoobjectiveaoshou ldaobeaoused firstaotoaoviewaoaaoslide,aoandaohowaotoaomanipulateaotheaoamountaoofaolightaothataopasse saothroughaothe slide.ao-aoans--useaotheaoirisaodiaphragmaotoaomanipulateaotheaolight -beginaowithao4xaolens -100xaoisaoforaooilaoemmersion Whyaodidaoweaouseaoaaonutrientaogelatinaodeep?ao-aoans--testaoforaoproteinaobreakdown Whyaodidaoweaoleaveaonutrientaogelatinaodeepaoataoroomaotemperature,aoinsteadaoofaousi ngaotheaoincubatorao-aoans-- itaobreaksaodownaobecauseaoofaotheaohighaotemp.aoofaotheaoincubator OnaoaaoPetriaodish,aoknowaotheaodifferentaomarginsaoofaoaaocolony,aoandaotheaodifferentao elevationsaoofaoa colony.ao-aoans--pageao 26 Onaoanaoagaraoslant,aoknowaotheaodifferentaogrowthaopatterns.ao-aoans--page.ao 43 algaeaomicroscopeaoslide.ao-aoans-- protozoaaomicroscopeaoslide.ao-aoans-- fungiaomicroscopeaoslide.ao-aoans-- gramaostainaomicroscopeaoslideao-aoans-- acidaofastaostainaomicroscopeaoslieao-aoans--