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MICROBIOLOGY LAB PRACTICAL EXAM
STUDY GUIDE
OcularaoLensaoofaoMicroscopeao-aoans-- RevolvingaoNosepieceaoofaoMicroscopeao-aoans-- ObjectiveaoLensaoofaoMicroscopeao-aoans-- StageaoofaoMicroscopeao-aoans-- IrisaoDiaphragmaoofaoMicroscopeao(oraoIrisaoDiaphragmaoLever)ao-aoans-- LampaoofaoMicroscopeao-aoans-- ArmaoofaoMicroscopeao-aoans-- SlideaoClipaoofaoMicroscopeao-aoans-- CourseaoFocusaoofaoMicroscopeao-aoans-- FineaoFocusaoofaoMicroscopeao-aoans-- RoundaoBacterialaoColonyao-aoans-- IrregularaoBacterialaoColonyao-aoans-- FilamentousaoBacterialaoColonyao-aoans-- RhizoidaoBacterialaoColonyao-aoans-- SmoothaoBacterialaoColonyaoMarginao-aoans-- LobateaoBacterialaoColonyaoMarginao-aoans-- ConvexaoColonyaoElevationao-aoans-- UmbonateaoColonyaoElevationao-aoans--
RaisedaoColonyaoElevationao-aoans-- PigmentedaoColonyao-aoans--COLOR DullaoColonyaoSurfaceao-aoans-- ShinyaoColonyaoSurfaceao-aoans-- DryaoColonyaoConsistencyao-aoans-- MoistaoColonyaoConsistencyao-aoans-- MucoidaoColonyaoConsistencyao-aoans-- OpaqueaoColonyao-aoans--NotaoSeeaoThrough TranslucentaoColonyao-aoans-- SedimentaoGrowthao-aoans--settlementaoataotheaobottomaoofaotheaoliquidaomedia FlocculentaoGrowthao-aoans--massesaoinaotheaosubsurfaceaoareaaoofaobrothaoculture FineaoTurbidityaoGrowthao-aoans--Cloudyaothroughout BrothaoSurfaceaoRingao-aoans-- Pellicleao-aoans--growthaoonaotopaoofaobrothaoculture TetradaoArrangmentao-aoans--inaogroupsaoofao 4 SarcinaaoArrangementao-aoans--inaogroupsaoofao 8 GramaoPostiveao-aoans--Purpleao--aoTHICKaocellaowall GramaoNegativeao-aoans--Pinkao--aoTHINaocellaowall NegativeaoStainao-aoans--stainsaoBACKGROUND AcidaoFastaoStainao-aoans--StainsaoFuchsiaao(pink)ao NONaoACIDaoFASTaoSTAINaoisaoBLUE/GREEN EndosporeaoStainao-aoans--CYTOPLASMaostainedaoRED SPORESaostainedaoDARKaoGREEN CapsuleaoStainao-aoans--
PositiveaoResultaoForaoCatalaseaoTestao-aoans--Bubbles NegativeaoResultaoForaoCatalaseaoTestao-aoans--NoaoBubbles PositiveaoResultaoForaoCoagulaseaoTestao-aoans--Plasmaaoformsaoaaoclot NegativeaoResultaoForaoCoagulaseaoTestao-aoans--Plasmaaoremainsaoliquid PositiveaoResultaoForaoGelatinaoLiquidificationaoTestao-aoans--GelatinaoLiquified NegativeaoResultaoForaoGelatinaoLiquidificationaoTestao-aoans--Gelatinaoremainedaosolid WhataoisaotheaoimportanceaoofaotheaoCoagulaseaoTest?ao-aoans-- ItaodistinguishesaoStaphylococcusaoaureusaofromaootheraoGramaopositiveaococci WhataoisaotheaoenzymeaousedaoinaotheaoGelatinaoLiquidificationaoTest?ao-aoans-- Gelatinase PositiveaoResultaoForaoHydrolysisaoofaoMilkaoProteinaoTestao-aoans-- clearingaoaroundaobacteria WhataoisaotheaoENZYMEaousedaoinaotheaoHydrolysisaoofaoMilkaoProteinaoTest?ao-aoans-- Casease WhataoisaotheaoSUBSTRATEaousedaoinaotheaoHydrolysisaoofaoMilkaoProteinaoTest?ao- aoans--Casein WhataodoesaotheaoPhenolaoRedaoFermentationaoTestaodetermine?ao-aoans-- Whetheraotheaocarbohydrateao(glucose,aolactose,aosucrose)aowasaofermented PositiveaoResultaoForaotheaoPhenolaoRedaoFermentationaoTestao-aoans-- AaoandaoA/Gao(acidao&aoacid/gas)ao=aoYELLOWaoFORaoACIDaoPRODUCTION NegativeaoResultaoForaotheaoPhenolaoRedaoFermentationaoTestao-aoans-- Redao(Noaocoloraochange) Howaodoaoyouaoknowaoifaothereaoisaoacidaoproduction?ao-aoans--YELLOW WhataoisaotheaopHaoIndicatoraousedaoinaotheaoPhenolaoRedaoFermentationaoTest?ao- aoans--PhenolaoRed PostiveaoResultaoforaoStarchaoHydrolysisaoTestao-aoans--clearingaoaroundaotheaobacteria NegativeaoResultaoforaoStarchaoHydrolysisaoTestao-aoans--noaoclearingaoaroundaobacteria
WhataoisaotheaoreactantaousedaoinaotheaoStarchaoHydrolysisaoTestaotoaodetectaotheaoprese nceaoofaostarch?ao-aoans--Gram'saoIodine PositiveaoMotilityaoTestao-aoans-- Raspberryaodiffuseaogrowthaoextendingaooutwardaofromaotheaostabaoline NegativeaoMotilityaoTestao-aoans-- Raspberryaocoloredaogrowthaoalongaotheaostabaolineaoonly PositiveaoUreaseaoTestao-aoans--Pinkao(Basic) NegativeaoUreaseaoTestao-aoans-- PhaseaoIaoNitrateaoReductionaoTestaoPostiveaoResultao-aoans--red PhaseaoIaoNitrateaoReductionaoTestaoNegativeaoResultao-aoans--noaochange PhaseaoIIaoNitrateaoReductionaoTestaoPostiveaoResultao-aoans--noaochange PhaseaoIIaoNitrateaoReductionaoTestaoNegativeaoResultao-aoans--pink PositiveaoResultaoForaoSulfuraoReductionaoTestao-aoans--BlackaoPrecipitate Whataoisaotheaoblackaoprecipitateaoformedaoinaoaaopositiveaosulfuraoreductionaotest?ao- aoans--FerricaoSulfide WhataodoesaoBloodaoTypingaoidentify?ao-aoans--theaopresenceaoofaoanaoAntigen Whataoisaoaaopositiveaoresultaoforaobloodaotyping?ao-aoans-- (Agglutination)aoClumpingaoinaotheaowellaoforaotheaoantigen/ antibody;aoRhaofactorao=ao+aobloodaotype Moldao--aoMACROscopicaoViewao-aoans--hairyaoandaofuzzy Moldao--aoMICROscopicaoViewao-aoans-- Yeastao--aoMACROscopicaoViewao-aoans--colorlessaoandaosmoothao-- aosimilaraotoaobacteria Yeastao--aoMICROscopicaoViewao-aoans--biggeraothanaobacteriaaoandaolarge Euglenaao--aoLocomotionaoToolao-aoans--Flagella Euglenaao--aoImageao-aoans--greenaoinaocolor
Aaopositiveaoindicationaoofaogasaoproductionaoisaoaao______________aoinaotheaotopaoofaot heaoDurhamaotubeao-aoans--bubble Starchaohydrolysisaoisaoaaotestaothataoisaoperformedaoinaoorderaotoaodistinguishaoamongao bacteriaaothataoproduceaotheaoenzymeao________________.ao-aoans--amylase TheaoUreaseaoTestaodeterminesaowhetheraoaaomicroorganismaocanaohydrolyzeaoureaaoint oao_____________aoandao_______________.ao-aoans--ammoniaaoandaocarbonaodioxide Kirby-BaueraoTestao-aoans-- aaotypeaoofaoantibioticaosensitivityaotestingaousedaotoaodetermineaotheaosusceptibilityaoofao bacteriaaotoaovariousaoantibiotics ZoneaoofaoInhibitionao-aoans-- indicatesaotheaodegreeaoofaosensitivityaoofaobacteriaaotoaoaaodrugaoORaotheaosizeaoofaothis aozoneaodependsaoonaohowaoeffectiveaotheaoantibioticaoisaoataostoppingaotheaogrowthaoofao theaobacterium SynergisticaoEffectao-aoans-- whenaotwoaodrugsaoareaostrongeraothanaooneaodrugaoaloneaoataokillingaobacteria UniversalaoDonorao-aoans--O- UniversalaoReceiverao-aoans--AB+ DNAaoisaoextractedaofromaohumanaocellsaoforaoaaovarietyaoofaoreasons.aoWithaoaaopureaos ampleaoofaoDNAaoyouaocanaotestao_______________,ao__________________,ao______ ___________aoorao_______________.ao-aoans-- aaonewbornaoforaoaaogeneticaodisease,aoanalyzeaoforensicaoevidence,aoDNAaoengineerin gaooraostudyaoaaogeneaoinvolvedaoinaocancer CocciaoMorphologyao-aoans--round BacilliaoMorphologyao-aoans--rods SpirillaaoMorphologyao-aoans--spirals DiploaoArrangementao-aoans--pairs StreptoaoArrangmentao-aoans--chains StaphyloaoArrangmentao-aoans--clusters Indicateaotheaomoleculeaoresponsibleaoforaoaaopositiveaocatalaseaotest.ao-aoans-- H2O2ao(HydrogenaoPeroxideao--aocausesaobubblesaoinaoaaopositiveaotest)
Useaoofaomicropippettorsao-aoans-- Micropipettorsaoareaotheaostandardaolaboratoryaoequipmentaousedaotoaomeasureaoandaotr ansferaosmallaovolumesaoofaoliquids. ResourceaoofaoHowaoToaoUse:aohttp://faculty.buffalostate.edu/wadswogj/courses/ bio211%20page/resources/micropipetting%20lab.pdf Theaoprimaryaostainaoinaogramaostainingaoisao________________.ao-aoans--crystalaoviolet Theaocounterstainaousedaoinaogramaostainingaoisao_____________.ao-aoans--safranin Yeastaometabolizesao_________________aofasteraothanaoglucose.ao-aoans--sucrose MediumaoofaoCoagulaseaoTestao-aoans--RabbitaoPlasma Catalaseaoisaoanaoexoenzyme,aothataoreactsaotoaoformao_______________aoandao_____ ______.ao-aoans--WateraoandaoOxygenaoGas BasisaoForaoCatalaseaoTestao-aoans--2H₂O₂ao-(Catalase)------------->ao2H₂Oao+aoO₂(g) FunctionaoofaoSDSaoinaoextractingaoDNAaofromaobacteriaao-aoans-- causesaotheaocellaowallsaoandaomembranesaotoaoburstaoandaotheaocontentsaoofaotheaobact eriaaoareaospilledaoout FunctionaoofaoproteinaseaoKaoinaoextractingaoDNAaofromaobacteriaao-aoans--removes/ cutsaoproteins FunctionaoofaocoldaoethanolaoinaoextractingaoDNAaofromaobacteriaao-aoans-- SinceaoDNAaoisaoinsolubleaoinaotheseaoalcohols,aoitaowillaoaggregateaotogether,aogivingaoa aopelletaouponaocentrifugationao(PrecipitateaoDNA) GelatinaoTestaoandaoMilkaoTestaoProductsao-aoans--polypeptidesaoandaoaminoaoacid StarchaoTestaoProductao-aoans--glucose __________________aoisaotheaomediumaoforaotheaosulfuraoreductionaotest.ao-aoans--SIM TwoaocharacteristicsaoofaoallaoProtistaaobacteria.ao-aoans---aounicellular
- aoeukaryotic Theaobubblesaoinaotheaocatalaseaotestaoareaocreatedaofromaotheao____________.ao- aoans--oxygenaogas Inaotheaocoagulaseaotest,aowhataoisaoagglutinating?ao-aoans--FibrinaoBarriers
TheaoKirby-BaueraoTestaotestsaoforao________________________.ao-aoans-- resistanceaoofaobacteria Ifaotheaozoneaoofaoinhibitionaoisaoveryaolarge,aowhataodoesaothataomeanaoaboutaoyouraobac teria?ao-aoans--Itaoisaoveryaosusceptibleaotoaotheaoantimicrobialao(dead) Ifaothereaoisaogrowthaoallaotheaowayaoupaotoaotheaoantimicrobialaodisk,aowhataodoesaothatao indicate?ao-aoans-- Theaobacteriaaoisaoveryaoresistantaobecauseaoitaogrewaonextaotoaotheaodisk Antimicrobialao-aoans--somethingaosyntheticaothataokillsaooraoinhibitsaomicroorganisms Antimicrobicsao-aoans--syntheticaodrugsaocapableaoofaokillingaomicroorganisms Antibioticsao-aoans--naturalaoformaoofaodefensesaousedaotoaokillaomicroorganisms IfaoitaoclumpsaoinaotheaoRhaowell,aoandaonowhereaoelse,aowhataoisaotheaobloodaotype?ao- aoans--O+ PositiveaoResultaoForaoBloodaoTypingao-aoans-- agglutinationaoofaoantibodiesaoandaoantigens Endosporesao-aoans--un- killableaoanaerobesaowithaoaaokeratinaoproteinaocoataothataoallowsaothemaotoaosurviveaoina oextremeaoheat,aocold,aooraootheraoconditions
