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Microscopy is the technical field of using microscopes to view samples & objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye)
Typology: Study notes
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3 structural parts of the microscope:
It carries the optical parts in the upper part of the microscope.
It also carries microscopic illuminators.
base and to the head and the eyepiece tube to the base of the microscope. It gives support to the head of the microscope and it is also used when carrying the microscope. Optical parts of a microscope and their functions:
This is the part used to look through the microscope. It’s found at the top of the microscope. Its standard magnification is 10x with an optional eyepiece having magnifications from 5X to 30X.
holder. It carries the eyepiece just above the objective lens. In some microscopes such as the binoculars, the eyepiece tube is flexible and can be rotated for maximum visualization, for variance in distance. For monocular microscopes, they are none flexible.
major lenses used for specimen visualization. They have a magnification power of 40x-100X. There are about 1- 4 objective lenses placed on one microscope, in that some are rare facing and others face forward. Each lens has its own magnification power.
revolving turret. It holds the objective lenses. It is movable hence it Cal revolve the objective lenses depending on the magnification power of the lens.
knobs that are used to focus the microscope. There are two types of adjustment knobs:
specimen is placed for viewing. They have stage clips that hold the specimen slides in place.
microscope stage, through which the transmitted light from the source reaches the stage.
microscopes light source , located at the base. It is used instead of a mirror. It captures light from an external source of a low voltage of about 100v.
used to collect and focus light from the illuminator into the specimen. They play a major role in ensuring clear sharp images are produced with a high magnification of 400X and above. The higher the magnification of the condenser, the more the image clarity.
iris. Its found under the stage of the microscope and its primary role is to control the amount of light that reaches the specimen. It’s an adjustable apparatus , hence controlling the light intensity and the size of the beam of light that gets to the specimen.
that moves the condenser up or down thus, controlling the focus of light on the specimen. Abbe Condenser – this is a condenser specially designed for high-quality microscopes, which makes the condenser to be movable and allows very high magnification of above 400X. High-quality microscopes normally have a high numerical aperture than objective lenses. The rack stop – It controls how far the stages should go preventing the objective lens from getting too close to the specimen slide which may damage the specimen. It is responsible for preventing the specimen slide from coming too far up and hitting the objective lens.