Microscopy Lecture note, Study notes of Biology

Microscopy is the technical field of using microscopes to view samples & objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye)

Typology: Study notes

2021/2022

Uploaded on 01/20/2024

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Microscope: Parts and Function
3 structural parts of the microscope:
1. Head - This is also known as the body.
It carries the optical parts in the upper
part of the microscope.
2. Base - It acts as microscopes support.
It also carries microscopic illuminators.
3. Arm This is the part connecting the
base and to the head and the eyepiece
tube to the base of the microscope. It
gives support to the head of the
microscope and it is also used when
carrying the microscope.
Optical parts of a microscope and
their functions:
Eyepiece also known as the ocular.
This is the part used to look through the
microscope. It’s found at the top of the
microscope. Its standard magnification
is 10x with an optional eyepiece having
magnifications from 5X to 30X.
Eyepiece tube it’s the eyepiece
holder. It carries the eyepiece just
above the objective lens. In some
microscopes such as the binoculars, the
eyepiece tube is flexible and can be
rotated for maximum visualization, for
variance in distance. For monocular
microscopes, they are none flexible.
Objective lenses These are the
major lenses used for specimen
visualization. They have a magnification
power of 40x-100X. There are about 1-
4 objective lenses placed on one
microscope, in that some are rare
facing and others face forward. Each
lens has its own magnification power.
Nose piece also known as the
revolving turret. It holds the objective
lenses. It is movable hence it Cal revolve
the objective lenses depending on the
magnification power of the lens.
The Adjustment knobs These are
knobs that are used to focus the
microscope. There are two types of
adjustment knobs:
- fine adjustment knobs
- coarse adjustment knobs.
Stage – This is the section in which the
specimen is placed for viewing. They
have stage clips that hold the specimen
slides in place.
- The most common stage is the
mechanical stage, which allows the
control of the slides by moving the
slides using the mechanical knobs on
the stage instead of moving them
manually.
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Microscope: Parts and Function

3 structural parts of the microscope:

1. Head - This is also known as the body.

It carries the optical parts in the upper part of the microscope.

2. Base - It acts as microscopes support.

It also carries microscopic illuminators.

3. Arm – This is the part connecting the

base and to the head and the eyepiece tube to the base of the microscope. It gives support to the head of the microscope and it is also used when carrying the microscope. Optical parts of a microscope and their functions:

 Eyepiece – also known as the ocular.

This is the part used to look through the microscope. It’s found at the top of the microscope. Its standard magnification is 10x with an optional eyepiece having magnifications from 5X to 30X.

 Eyepiece tube – it’s the eyepiece

holder. It carries the eyepiece just above the objective lens. In some microscopes such as the binoculars, the eyepiece tube is flexible and can be rotated for maximum visualization, for variance in distance. For monocular microscopes, they are none flexible.

 Objective lenses – These are the

major lenses used for specimen visualization. They have a magnification power of 40x-100X. There are about 1- 4 objective lenses placed on one microscope, in that some are rare facing and others face forward. Each lens has its own magnification power.

 Nose piece – also known as the

revolving turret. It holds the objective lenses. It is movable hence it Cal revolve the objective lenses depending on the magnification power of the lens.

 The Adjustment knobs – These are

knobs that are used to focus the microscope. There are two types of adjustment knobs:

  • fine adjustment knobs
  • coarse adjustment knobs.

 Stage – This is the section in which the

specimen is placed for viewing. They have stage clips that hold the specimen slides in place.

  • The most common stage is the mechanical stage , which allows the control of the slides by moving the slides using the mechanical knobs on the stage instead of moving them manually.

Microscope: Parts and Function

 Aperture – This is a hole on the

microscope stage, through which the transmitted light from the source reaches the stage.

 Microscopic illuminator – This is the

microscopes light source , located at the base. It is used instead of a mirror. It captures light from an external source of a low voltage of about 100v.

 Condenser – These are lenses that are

used to collect and focus light from the illuminator into the specimen. They play a major role in ensuring clear sharp images are produced with a high magnification of 400X and above. The higher the magnification of the condenser, the more the image clarity.

 Diaphragm – it’s also known as the

iris. Its found under the stage of the microscope and its primary role is to control the amount of light that reaches the specimen. It’s an adjustable apparatus , hence controlling the light intensity and the size of the beam of light that gets to the specimen.

  • For high-quality microscopes, the diaphragm comes attached with an Abbe condenser and combined they are able to control the light focus and light intensity that reaches the specimen.

 Condenser focus knob – this is a knob

that moves the condenser up or down thus, controlling the focus of light on the specimen.  Abbe Condenser – this is a condenser specially designed for high-quality microscopes, which makes the condenser to be movable and allows very high magnification of above 400X. High-quality microscopes normally have a high numerical aperture than objective lenses.  The rack stop – It controls how far the stages should go preventing the objective lens from getting too close to the specimen slide which may damage the specimen. It is responsible for preventing the specimen slide from coming too far up and hitting the objective lens.