Osmosis in Eggs Validation, Exercises of Biology

This document is a Year 11 ATAR Biology practical worksheet focused on osmosis and cell membranes. It investigates how water moves across a selectively permeable membrane using eggs as a model system. The resource includes background theory on tonicity (hypertonic, hypotonic, isotonic), osmosis, and membrane structure. The booklet is structured into two parts: Practical Set-Up (aim, method, diagrams, variables, hypothesis) and Results & Conclusions (data collection, graphing, analysis, and evaluation). Students predict outcomes, identify variables, construct tables and graphs, and explain results using scientific terminology. It also develops skills in experimental design, data analysis, and identifying errors. Likely created by a Biology teacher, it supports understanding of diffusion and prepares students for ATAR assessments.

Typology: Exercises

2025/2026

Uploaded on 03/17/2026

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Cellular transport – Osmosis
In class practical – Osmosis in Eggs Practical & Validation
Questions
Investigating osmosis and cell membranes from observing the effects of
different concentrations
Understanding and using appropriate terminology to describe the
movement of water molecules across the selectively permeable cell
membrane
Background Information:
There have been many different models of the cell membrane
proposed to explain observations of cells in different
environments and results of investigations. Cell membranes are
too thin to observe them directly, but it is possible to infer cell
membrane structures from the reactions to different materials
and different conditions.
Terminology:
Tonicity is the relativeconcentration ofsolutes dissolved in solution
which determine the direction and extent ofdiffusion across a selectively
permeable membrane. It is commonly used when describing the response
ofcells immersed in an external solution. (Intracellular fluid and
extracellular fluid)
Tonicity Description Diagram explaining
osmotic flow
Hypertonic A hypertonic solution
has a greater
concentration
ofsolutes than
another solution.
Hypotonic A hypotonic solution
has a lower
concentration of
solutes than another
solution.
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Cellular transport – Osmosis In class practical – Osmosis in Eggs Practical & Validation Questions Investigating osmosis and cell membranes from observing the effects of different concentrations Understanding and using appropriate terminology to describe the movement of water molecules across the selectively permeable cell membrane Background Information: There have been many different models of the cell membrane proposed to explain observations of cells in different environments and results of investigations. Cell membranes are too thin to observe them directly, but it is possible to infer cell membrane structures from the reactions to different materials and different conditions. Terminology: Tonicity is the relative concentration of solutes dissolved in solution which determine the direction and extent of diffusion across a selectively permeable membrane. It is commonly used when describing the response of cells immersed in an external solution. (Intracellular fluid and extracellular fluid) Tonicity Description Diagram explaining osmotic flow Hyper tonic A hypertonic solution has a greater concentration of solutes than another solution. Hypo tonic A hypotonic solution has a lower concentration of solutes than another solution.

Iso tonic A solution is isotonic when its effective osmoles ( Osm/L) concentration is the same as that of another solution. Effect of osmotic pressure on red blood cells in the human body. This task is testing your ability to make observations and use them to demonstrate your understanding of osmosis. It is also to assess your understanding of variables and experimental design in a formative way. You will be able to refer to your notes and your textbook to help you to complete this part of the booklet – Part 1: Practical Set Up. Ensure that you use pencil for diagrams and that any straight lines are ruled. In a test situation, marks will be deducted for free-hand drawings and those done in pen.

Distilled Water Isotonic Saline (4 marks)

3. What is the INDEPENDENT variable in this experiment? _____________________________________________________________________ (1 mark) 4. What is the DEPENDENT variable in this experiment? _____________________________________________________________________ (1 mark) 5. List three other variables that are CONTROLLED in this experiment. a. _______________________________________________________________ b. _______________________________________________________________ c. _______________________________________________________________ (3 marks) 6. Write a hypothesis that could be used for this experiment. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (2 marks)

Part 2: Collection of Results and Conclusions Carefully observe the eggs and record any new information in your table. Graph ( marks) Construct a graph of your results in the space below. Consider carefully the data that you will choose to display. Include a title, labelled axis with units, good scales on your axis, correct plotting and the correct type of graph.

_____________________________________________________________________

_____________________________________________________________________

_____________________________________________________________________

_____________________________________________________________________

(2 marks)

5. Use the space below to construct a diagram to explain osmosis and how it works with the concentration gradient. Be sure to use words to explain what is occurring. (4 marks) 6. List THREE sources of experimental error in this practical and explain how they could have been better controlled. a. _______________________________________________________________ _______________________________________________________________ b. _______________________________________________________________ _______________________________________________________________ c. _______________________________________________________________ _______________________________________________________________ (3 marks) Conclusion: (2 marks)

Refer to your hypothesis to help you to write a few sentences to summarise the experiment.