pH theory + biochemical calculations, Exercises of Biochemistry

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What is the molarity of Baie H2SOs solution? Density (96% H2SOz) = 1.86 g/cm’. Mr(HsS0.) = 98 % ‘/. — 0.46 M~= 18609 7 sume At = 0.46%|/860 - IF89.6 = 1988-6 . 18.2 m0t) Micon 186 QB Rs Bee «18.290 Di\yxtTOnS > 4 5.5.2. Penicillin vial contains 1 500 000 IU (IU = international unit) of penicillin. The patient should be given 1 200 000 IU of penicillin in one dose. How many ml of penicillin solution do you put into the syringe when penicillin in the vial is diluted a) by 10 ml of dilution solution, b) by 5 ml of dilution solution? 0 wee = 150000 1Yéme —99% py 1800600 agp 900 IW /mt Hier yx (200000 Bm = 5 1 uml x 5.5.3. A 3% hydrogen peroxide solution 1s used to disinfection. How many ml of a 30% H20z solution is needed to prepare 500 ml of 3% hydrogen peroxide solution? CeCe VatO-4- S00mLx0.03 Va + SOME 5.5.4. How would you prepare 150 g of a 5% NaOH solution from 10% stock NaOH solution? Nook: O.LM 7 NOOK: OOSKIND “—~ Hima.s ASML 74.59 Ms FC / —- cuareantne $<(7) sone (mass )— fonhKs t otal MOS -(1) tones $ OONGHY pe te Mipi= Mp —— 5.5.6. 25 ml of 90% solution was mixed with 60 ml of 40% solution to otiain G09) soiton What volume of 30% solution do you obtain and how many milliliters of water do yoheed? MPs + MaPa = plmeMr) tore 0 gor ism 9s -O0%+ 60-04. 0.3lv) 420 7 an a N-ISSML 7% ISS-QS = OM! does) rao" Pe ay nd yu So 5.5.9. What volume of water (in milliliters) should be added to/200 ml of 68% HNOs (density = 1.4 * g/cm*) to make 10% solution? Myo, . 900 x LU: 1809 mn is ae 0.1 Manac = 140-4 m = 220% 6.08 = 0,49 in m Mena, = 12049 HO 1904 - 380 = (G24 mL Pron AD). SPechophotomenry 5.6.3.5 mg/l drug standard solution reads absorbance A = 0.4. Absorbance of an unknown sample of drug is 0.25. What is the drug concentration of the unknown sample? c-smgn 4-04 Hye . kK: 3.126 myie } A. 0.28 0.28 — * 5.6.4. Calculate the molarity of vitamin D2 solution if its € = 1.84 x 104, length of cuvette = 1. cm and its transmittance is 0.4. L> Az -logt a cx SAOxlO'S i tng to¥e > QI Az €x |¥C OM: 1-QYsIDNxAx Cc N= €- lee pH 5.7.9. 50 mL of a solution contains 4 mg of NaOH. Calculate the pH value “of this ‘Solution. i laled 4mq —> 0.0049 n+ 0.004 . 9.0004 _ 9.007 mul POH: -109(0.002)= 2:4 pHe ID, 0.05 —~_Le pent 5.7.10. 50 cm of a 0.2 M NaOH solution was diluted to the final volume of 800 ml. What is the pH value of this solution? 5040.2, ¥0.2 CMe Cay AF Rs OOS BDH 1.90 — le) x 5.7.11. Calculate the pH value of a 0.08% NaOH solution. Mr(NaOH) = 40 ky E> 0-08 por (00 ML —> 0-8 4/7 30 08 pOH « - 109 (0.02) « 16a | pas [2-31 ¢~ Sos 007 lo) NO UNIFY OH uaak woges } Omid gg tv 5.8.2. 25.0 ml of a CHsCOOH solution has pH = 3.9. Calculate the weight of CHsCOOH in this solution ) (in milligrams). pKa = 4.75, Mr(CHsCOOH) = 60 + . VK t 8 3x0" x Cut< 1077. 1.26410" tH") gael’ a ¥ 0.025 ~ Ss Me nelle = AZHxID3g = Astmg/ * : 2 23x 5.8.3. Calculate the pH value of 0.5% acetic acid solution. 1 = 60.05, density = 0.998 g/ml and pKa = 4.76 per 100 Ll : : gait pH = (4.46 - log c\ - aar* ce yz 1004. (ame 0.6 371 100-7 2 = O% x Aovo = 4. ZT aaagi 60.08 0998 sep 5 OREN Ag (HPO / H:PO+) found in cytosol, if of HsPOr + HPO + H"is 7.0, frio. SS / 5.9.5. Calculate the ratio of pho utfer components (HPO<* / H:POx) found in eytosol, if Intracellular pH = 6.87 pK cor Oe + Hi