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Primary Fermentation
GrapeMaturity Pre-Ferm 1°^ 2°^ Aging^ Wine Stabilization^ Bottling
Harvest (pH, TA, Brix, MOG)^ Yeast Inoculation MLB Inoculation^ Add Molecular SO
2
Sugar Decline Malic Decline VA and FSO
2 Big-3^ DO
Master Blend Completed Stabilization Complete
TIME
Clean Winemaking Stage Diagram
Created by Bryan Avila : 2008
Grape Notes
Following veraison
Grape Maturity Monotoring Stage
QC
Milestones
Gates Stylistic^ TempNutrients RS-Enz Mal-Enz (^) Stylistic Stable
Bottled Wine
SO2/BP
Time (Not to Scale)
Introduction
- The goal of fermentation is to convert sugar into ethanol with a minimum of undesired by- products
- Optimize fruit character, mouthfeel and tastes
- Theoretically 180 grams of sugar will convert to 92 grams of ethanol, 51% of weight.
- About 5% of sugar goes to other metabolic by- products that can contribute the wine’s complexity
Introduction
- Approximately 1.75 Brix = 1% Alcohol (v/v)
- 1/1.75 = 0.57 (aka conversion rate)
- Potential Alc = approx 0.57 x Brix
- Conversion rate varies depending on amount of non-sugar solids
- Less ripe fruit has a higher degree of non- sugar solids (They are very high in acids)
- Ethanol yield also depends on fermentation temp EtOH losses also come from volatilization
Wine Yeast
- Louie Pasteur found that winemaking was performed by yeast, not magic.
- Development of microbiological practices and experimentation of with pure yeast cultures have led to cleaner fermentations
- Inoculations with cultured yeast out compete wild microbes by sheer numbers and through breeding
Cultured Wine Yeast Attributes
- Tolerance to vinification conditions
- Alcohol, SO2, Temperature
- Different By-products
- Less Sulfides, VA, more esters, glycerol, etc.
- Ability to ferment to dryness
- Flocculation leading to better formation of lees
- Fermentation kinetics – rate of fermentation
Killer Yeast
- Mutated strain of yeast that releases toxins harmful to it’s own strain
- Toxin is a protein
- Can result in stuck fermentation
Fermentation
- Microbial cell growth follow a similar growth curve
Inoculation
- Performed when a pure culture is added to wine
- Liquid vs. active dry
- Inoculums are added to out compete wild strains through breeding and pure numbers (10^6)
- Rehydrating yeast goals
- Rehydrate yeast being careful not to grind yeast cells by harsh stirring
- Allow for temperature acclimatization
- Allow starter to become metabolically active
White Wine Fermentation
Common problems during Fermentation
- Ethyl Acetate – spoilage microbes, Wild yeast
- Sulfides – Nutrient deficiency is common but only one of many origins
- Sluggish/stuck fermentation – nutrition problems, extreme temp, spoilage microbes(toxins), ethanol toxicity
Ethanol Toxicity
Fermentation Tanks
What’s happening in the fermenter?
Fermentation Monitoring Temperature Control Gas Venting
Sanitary Design
Solids Handling
Engineering & Construction
- Conversion of grape sugars to ethanol
- Fermentation produces heat
- Yeast produce carbon dioxide gas
- Yeast are competing against other wild microorganisms
- Tanks hold several tons of must or juice
- Color and other phenolics are being extracted from skins in red fermentation