Malolactic Fermentation - Wine - Lecture Slides, Slides of Winemaking

Download Malolactic Fermentation - Wine - Lecture Slides and more Slides Winemaking in PDF only on Docsity!

Malolactic Fermentation

Overview

• QA Overview
• Initial Measurements
• ML Process
• Chemistry
• Methods

Common Initial Measurements

• pH
• Titratible Acidity (TA)
• Malic acid
• VA

Chemistry

• Transforms malic acid into lactic acid
• Lactic acid is less acidic than malic acid
• May see a reduction in TA between 1 – 3 g/L
• MLF adds other metabolic products that may contribute to complexity such as diacetyl
• MLF can be inoculated or may start spontaneously
• Presence of Lactic acid will show history of MLF activity

Common monitoring methods

• Visual observation  bubbles in the fermentation lock (good to 100mg/l)
• Paper chromatography  qualitative method
• Enzymatic  Precise method
• HPLC

Malic by Paper Chromatography

1. placing a small dot or line of sample solution onto a strip of chromatography paper.
2. The paper is placed in a jar containing a shallow layer of solvent and sealed.
3. solvent rises through the paper, meets the sample mixture which starts to travel up the paper with the solvent.

ML by Chromatography Set-up

Malic by Enzymatic

• Draw