Creative BioMart's Whole-Genome Bisulfite Sequencing for Methylation Analysis, Slides of Biology

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Single-cell WGBS Service

DNA methylation is an important epigenetic marker information, and the methylation level data of all

cytosine residues in the genome-wide range is of great significance for the epigenetic space-time

specificity studies. The analysis of methylation modification patterns of specific species will surely have a

landmark significance in epigenomics research, and lay a foundation for the study of basic mechanisms

such as cell differentiation, tissue development, animal and plant breeding, as well as human health and

diseases. Based on the next-generation sequencing platform, Creative BioMart performs whole-genome

bisulfite sequencing (WGBS) with low-cost, high-efficiency and high-accuracy.

What Is Whole-Genome Bisulfite Sequencing?

Whole-genome bisulfite sequencing (WGBS) is an effective and reliable strategy for the identification of

methylated cytosines across the genome. This is a DNA methylation analysis method based on bisulfite

treatment and sequencing. For the detection of sequences, this technique combines next-generation

sequencing of high accuracy, high throughput and excellent bioinformatic analysis platform. The next-

generation sequencing allows for the analysis of 5mC at single-nucleotide resolution across the genome.

The principle of WGBS is to utilize bisulfite process the genome, convert the unmethylated cytosine into

uracil and after PCR amplification, uracil transforms into thymine, which is distinguished from the

original methylated cytosine. Combined with high-throughput sequencing technology, the methylation

of CpG/CHG/CHH loci can be determined by comparing with the reference sequence. This method is

capable of testing approximately 90% of the cytosines in the genomes studied so far.

Workflow of Whole-Genome Bisulfite Sequencing at Creative BioMart Creative BioMart strictly controls each step in the WGBS workflow to ensure access to comprehensive, high-quality genome-wide methylation sequencing data. Our post-bisulfite construction strategy is that DNA samples are treated with bisulfite prior to introduction of sequencing adapters, and then sequencing. Please contact us for more details about our whole-genome bisulfite sequencing (WGBS) service. Our experts will help design an optimal solution for your project and trouble-shoot for you throughout the whole process. If you would like to obtain genome-wide methylation information at single-cell level, our single-cell WGBS service will meet your needs. Reference

1. Miura, F.; et al. Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging. Nucleic Acids Research. 2012, 40(17): e136.
2. Raine, A.; et al. SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing. Nucleic Acids Research. 2016, 45(6): e36.

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