RNA Methylation Analysis: Understanding and Detecting this Epigenetic Modification, Slides of Biology

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RNA Methylation Analysis

Creative BioMart is a leading provider of comprehensive, high quality RNA methylation analysis and is

proud of offering and tailoring most types of sequencing services to meet the research objectives and

budget of our clients. We hope to partner with you to explore new frontiers in biological science and

accelerate your discovery.

As one of the most important content of epigenetic research, RNA methylation refers to a reversible and

post-transcriptional methylation modification occurring at different positions in RNA molecules and it

affects several biological processes. N6-methyladenosine (m6A) and C5-methylcytidine (5mC) are two

post-transcriptional modifications of RNA commonly found in eukaryotes. RNA methylation plays a key

role in gene expression regulation gene expression, splicing, RNA editing, RNA stability, controlling

lifespan and mRNA degradation of mRNA. Compared to DNA methylation, RNA methylation is more

complex and diverse, and is ubiquitous in various eukaryotic cells. Due to the lack of effective detection

methods, related studies are mostly limited to non-coding tRNA and rRNA, or a small number of coding

transcripts. At the same time, most of RNA methylation functions are unknown.

RNA Methylation Study Procedures To facilitate the detection of RNA methylation, Creative BioMart has established a series of methods for analyzing base methylation in RNA using bisulfite sequencing, MeRIP-seq technology and so on. Treatment of RNA with bisulfite causes the chemical deamination of nonmethylated cytosines to uracil, while methylated cytosines remain unaffected. cDNA synthesis followed by PCR amplification and DNA sequencing allows investigators to obtain the level of RNA methylation in tRNAs and rRNAs. By using high-throughput sequencing approaches, this protocol could enable the characterization of 5mC methylation patterns in RNA molecule. In addition, methylated RNA-specific antibodies are incubated with randomly interrupted RNA fragments, and methylated fragments are captured for sequencing. At the same time, a control sample needs to be sequenced in parallel to eliminate the background during the capture of methylated fragments. The two types of sequence fragments are then compared (or localized) to the reference genome/transcriptome to detect methylation level of m6A.

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Excerpt from: https://www.creativebiomart.net/epigenetics/services/rna- methylation-analysis/