Targeted Bisulfite Sequencing: A Precise Method for DNA Methylation Analysis, Slides of Biology

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Targeted Bisulfite Sequencing

With the advent and popularity of next-generation sequencing (NGS) technology, there are now many ways to

analyze DNA methylation across the entire genome or large regions of the genome. These approaches open the

possibility of discovering studies that quantify DNA methylation patterns and differences in DNA methylation. In

addition to these hypothesis-generating methods, there is a great need for a targeted/hypothesized testing method

for DNA methylation quantification. As a supplier dedicated to epigenetic research, Creative BioMart provides our

customers with highly customized and cost-effective targeted bisulfite sequencing service, which allows the DNA

methylation status analysis of targeted genomic regions and is very suitable for the analysis of large numbers of

samples.

What Is Targeted Bisulfite Sequencing?

Targeted bisulfite sequencing is a technique based on bisulfite sequencing for assessing site-specific DNA

methylation changes. This technique can accurately and effectively target any interested subset of the genome for

high-resolution cytosine methylation analysis. For the purpose of “targeting”, hybridization or region-specific PCR

amplification can be employed. In targeted bisulfite sequencing based on a hybridization step, bisulfite-converted

DNA can be hybridized to a pre-designed oligonucleotide capture array to enrich regions of interest for targeted

bisulfite sequencing, such as CpG islands, promoters, and other important methylated regions. Targeted bisulfite

sequencing based on region-specific PCR amplification is performed through the bisulfite treatment of the DNA,

followed by PCR amplification of the targeted region, library construction and the sequencing of the amplicon

region, facilitating the methylation analysis of the single-base resolution. Specific primers are designed for regions

of interest and cytosine methylation changes are evaluated in these regions.

Workflow of Targeted Bisulfite Sequencing at Creative BioMart

Creative BioMart strictly controls each step in the targeted bisulfite sequencing workflow to

ensure access to unsurpassed data quality. In the process of targeted bisulfite sequencing based

on hybridization, we utilize both commercially designed capture arrays and custom-designed

capture libraries to fully meet your research objectives.

In the process of targeted bisulfite sequencing based on region-specific PCR

amplification, the genomic DNA is bisulfite converted and multiplex amplified using

specifically designed and validated primers, and the amplicons are pooled through

barcoding and adapterization. The amplicon libraries are then subjected to sequencing.

Thank you!

Excerpt from:

https://www.creativebiomart.net/epigenetics/services/

targeted-bisulfite-sequencing/