Overview of Creative Biostructure's MagHelix™ SPR Services in Drug Discovery, Slides of Biology

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spr fragment based screening

Optical biosensors have been utilized widely in many links and areas of drug discovery, such as target

identification, ligand screening, lead compound selection, early ADME as well as manufacturing quality

control. Among them, surface plasmon resonance (SPR) technology is a rapidly developing approach for

determining binding events, which can characterize target protein-ligand interactions in real

time. Creative Biostructure has extensive expertise and experience in SPR technology, and we can exploit

this technology to provide sensitive, rapid, and reliable assays to help you measure real-time binding and

kinetic information of ligands against specific targets. Our MagHelix™ SPR is a customizable solution that

optimizes procedure for specific drug discovery projects, from experimental design to downstream

applications.

Brief Introduction to Surface Plasmon Resonance (SPR)

SPR utilizes an optical approach to measure the refractive index change of the medium in close vicinity of

a metal surface which can be applied to monitor the binding of ligands to receptors immobilized on the

metal surface. This takes advantage of the phenomenon of surface plasmon generation in the thin metal

films and the total internal reflection of light at the surface-solution interface, producing an

electromagnetic film or evanescent wave which extends a short distance into the solution. Usually, the

target protein is immobilized on the surface of the metal sensor, and then, the probe solution flows over

the surface of the target, and if binding occurs, the probe will induce an increase in the refractive index.

Therefore, SPR can determine the changes in reflected light before and after the probe binds to the target.

Briefly, the advantages of SPR include: 1) It is highly sensitive and suitable for detecting weak target-

ligand interactions mediated by fragments; 2) Without labels and the detection of false positives caused

by fluorescence quenching can be excluded; 3) Relatively small quantities of samples can be used for

measuring real-time quantitative kinetics and binding affinity; 4) The medium-throughput mode makes

it available as a tool for screening ligands. However, information about the binding site or

pharmacophore of a ligand cannot be obtained.

Advantages of our MagHelix™ Surface Plasmon Resonance (SPR) services:

Based on powerful Biacore and Fortebio systems, we have provided customers with many high-quality

solutions for monitoring interactions between various proteins, DNA/RNA as well as small/complex

molecules.

We have many years of experience in monitoring biomolecular interactions such as protein-small

molecule, protein-peptide, protein-protein, antigen-antibody, and so on.

We can combine SPR technology with fragment-based screening approach for medium-throughput

screening of chemical libraries.

The next-generation SPR instruments enable us to analyze the ligand binding to membrane proteins in

a more native environment.

Using SPR technology to screen and characterize antibodies to understand their affinity and kinetic

characteristics.

At Creative Biostructure , we can offer corresponding compound libraries or fragment libraries for ligand

screening, and we also provide other

biophysical solutions to characterize the binding kinetics of hit compounds or fragments. Want to know

more? Please do not hesitate to contact us for more information.

References

1.Cooper M A. Optical biosensors in drug discovery. Nature reviews Drug discovery. 2002, 1(7): 515-528.

2.Patching S G. Surface plasmon resonance spectroscopy for characterisation of membrane protein-

ligand interactions and its potential for drug discovery. Biochimica et Biophysica Acta (BBA)-Biomembranes.

Related Services:

MagHelix™ Bio-layer Interferometry (BLI)

MagHelix™ Saturation Transfer Difference (STD) NMR

MagHelix™ Co-crystallization and Soaking

MagHelix™ Differential Scanning Calorimetry (DSC)

MagHelix™ Dynamic Light Scattering (DLS)