I. Obtención de secuencias para el alineamiento múltiple utilizando BLAST
>sp|P20472|PRVA_HUMAN Parvalbumin alpha OS=Homo sapiens GN=PVALB PE=1 SV=2
MSMTDLLNAEDIKKAVGAFSATDSFDHKKFFQMVGLKKKSADDVKKVFHMLDKDKSGFIE
EDELGFILKGFSPDARDLSAKETKMLMAAGDKDGDGKIGVDEFSTLVAES
>sp|P80079|PRVA_FELCA Parvalbumin alpha OS=Felis catus GN=PVALB PE=1 SV=2
MSMTDLLGAEDIKKAVEAFTAVDSFDYKKFFQMVGLKKKSPDDIKKVFHILDKDKSGFIE
EDELGFILKGFYPDARDLSVKETKMLMAAGDKDGDGKIDVDEFFSLVAKS
>sp|P02627|PRVA_PELES Parvalbumin alpha OS=Pelophylax esculentus PE=1 SV=1
PMTDLLAAGDISKAVSAFAAPESFNHKKFFELCGLKSKSKEIMQKVFHVLDQDQSGFIEK
EELCLILKGFTPEGRSLSDKETTALLAAGDKDGDGKIGVDEFVTLVSES
>sp|P02626|PRVA_AMPME Parvalbumin alpha OS=Amphiuma means PE=1 SV=1
SMTDVIPEADINKAIHAFKAGEAFDFKKFVHLLGLNKRSPADVTKAFHILDKDRSGYIEE
EELQLILKGFSKEGRELTDKETKDLLIKGDKDGDGKIGVDEFTSLVAES
>sp|P43305|PRVU_CHICK Parvalbumin, thymic CPV3 OS=Gallus gallus PE=1 SV=2
MSLTDILSPSDIAAALRDCQAPDSFSPKKFFQISGMSKKSSSQLKEIFRILDNDQSGFIE
EDELKYFLQRFECGARVLTASETKTFLAAADHDGDGKIGAEEFQEMVQS
>sp|Q91482|PRVB1_SALSA Parvalbumin beta 1 OS=Salmo salar PE=1 SV=1
MACAHLCKEADIKTALEACKAADTFSFKTFFHTIGFASKSADDVKKAFKVIDQDASGFIE
VEELKLFLQNFCPKARELTDAETKAFLKAGDADGDGMIGIDEFAVLVKQ
>sp|P02619|PRVB_ESOLU Parvalbumin beta OS=Esox lucius PE=1 SV=1
SFAGLKDADVAAALAACSAADSFKHKEFFAKVGLASKSLDDVKKAFYVIDQDKSGFIEED
ELKLFLQNFSPSARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKA
>sp|P02620|PRVB_MERME Parvalbumin beta OS=Merluccius merluccius PE=1 SV=1
AFAGILADADITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFVEE
DELKLFLQNFSAGARALTDAETATFLKAGDSDGDGKIGVEEFAAMVKG
>sp|P02622|PRVB_GADMC Parvalbumin beta OS=Gadus morhua subsp. callarias PE=1 SV=1
AFKGILSNADIKAAEAACFKEGSFDEDGFYAKVGLDAFSADELKKLFKIADEDKEGFIEE
DELKLFLIAFAADLRALTDAETKAFLKAGDSDGDGKIGVDEFGALVDKWGAKG
II. Alineamiento múltiple de secuencias utilizando ClustalW.
Sequence type explicitly set to Protein
Sequence format is Pearson
Sequence 1: sp|P20472|PRVA_HUMAN 110 aa
Sequence 2: sp|P80079|PRVA_FELCA 110 aa
Sequence 3: sp|P02627|PRVA_PELES 109 aa
Sequence 4: sp|P02626|PRVA_AMPME 109 aa
Sequence 5: sp|P43305|PRVU_CHICK 109 aa
Sequence 6: sp|Q91482|PRVB1_SALSA 109 aa
Sequence 7: sp|P02619|PRVB_ESOLU 107 aa
Sequence 8: sp|P02620|PRVB_MERME 108 aa
Sequence 9: sp|P02622|PRVB_GADMC 113 aa
Start of Pairwise alignments
Sequences (1:2) Aligned. Score: 87.2727
Sequences (1:3) Aligned. Score: 67.8899
Sequences (1:4) Aligned. Score: 62.3853
Sequences (1:5) Aligned. Score: 52.2936
Sequences (1:6) Aligned. Score: 50.4587
Sequences (1:7) Aligned. Score: 56.0748
Sequences (1:8) Aligned. Score: 50
Sequences (1:9) Aligned. Score: 49.0909
Sequences (2:3) Aligned. Score: 64.2202
Sequences (2:4) Aligned. Score: 61.4679
Sequences (2:5) Aligned. Score: 51.3761
