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Exercise 8 : Chemical and Physical Processes of Digestion Activity 2 : Exploring Amylase Substrate Specificity Name: GABRIELA ELIZABETH VENTURA Date: 18 July 2021 Session ID: session-10efcf03-ecb7-63f2-ef17-cd4c6aa0964b
You scored 100% by answering 5 out of 5 questions correctly.
Predict Question Stop & Think Questions Which of the following is true of enzymes and substrates? You correctly answered: Enzymes are specific about the substrates they can act upon.
Which of the following is/are reducing sugars? You correctly answered: both glucose and maltose.
Cellulose and starch are both You correctly answered: polymers of glucose and polysaccharides.
Proteins and peptides are formed by joining amino acids with a special type of covalent bond called a peptide bond. Which of the following enzymes do you think would be specific for a peptide bond? You correctly answered: peptidase.
The Benedict's assay tests for the presence of You correctly answered: reducing sugar.
Predict Question: Do you think test tube 3 (amylase, cellulose, pH 7.0 buffer) will show a positive Benedict's test? Your answer: no.
1 You included tube 2 (amylase, glucose, pH 7.0 buffer) to
Experiment Data Tube No. Reagent 1 Reagent 2 Reagent 3 Time (min) Temp (°C) IKI Benedict's 1 Starch Amylase pH 7. Buffer
2 Amylase Glucose pH 7. Buffer
3 Amylase Cellulose pH 7. Buffer
4 Cellulose pH 7. Buffer Deionized Water
5 Peptidase Starch pH 7. Buffer
6 Bacteria Cellulose pH 7. Buffer
You scored 100% by answering 4 out of 4 questions correctly. You correctly answered: see what a positive Benedict's test should look like. Which tube is included to detect contaminating amylase in the buffer or in cellulose? You correctly answered: None of the above.
What is the usual substrate for the pancreatic enzyme peptidase? You correctly answered: peptides.
The substrate(s) for amylase is/are You correctly answered: starch.
The results of tube 5 (peptidase, starch, pH 7.0 buffer) demonstrated that You correctly answered: peptidase does not digest starch.
Why was the cellulose in tube 6 (bacteria, cellulose, pH 7.0 buffer) hydrolyzed to glucose? You correctly answered: The bacterial suspension contained the enzyme cellulase, which digested the cellulose.
Which tube allows you to conclude that the buffer was not contaminated with amylase?