BACTERIAL CELL STRUCTURE & FUNCTION, Exams of Medicine

BACTERIAL CELL STRUCTURE & FUNCTION BACTERIAL CELL STRUCTURE & FUNCTION

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2025/2026

Available from 06/27/2026

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BACTERIAL CELL STRUCTURE &
FUNCTION
what are the 5 major groups of microorganisms? - ANSWER-1) bacteria (prokaryote)
2) fungi (eukaryote) -> yeast & mold
3) viruses
4) Protozoa (eukaryote)
5) Algae
the cytoplasmic membrane is a phospholipid bilayer that .... - ANSWER-1) acts as a
barrier to the environment
2) facilitates transport in and out of the cell
3) has extensive metabolic activity
What is peptidoglycan? - ANSWER-a network of sugar polymers cross-linked by
polypeptides (NAM-NAG)
Which type of bacteria contain a very thick layer of peptidoglycan? - ANSWER-gram
positive
which type of bacteria contain an inner and an outer membrane? - ANSWER-gram
negative
Lipopolysaccharide (LPS) - ANSWER-Molecule that makes up the outer layer of the
outer membrane of Gram-negative bacteria
O-antigen - ANSWER-immune activator from LPS that is responsible for many signs of
inflammatory syndrome
what are the three bacterial shapes? - ANSWER-bacillus (rod)
coccus (sphere)
spirillus (spiral)
what are 4 types of special bacteria? - ANSWER-1) naturally variant streptococci
2) bacterial L forms
3) bacteria that naturally lack a cell wall
4) obligate intracellular bacteria
bacterial L forms - ANSWER-temporary artificially induced variants of bacteria that lose
their cell wall due to environmental factors such as abx exposure
obligate intracellular bacteria - ANSWER-bacteria that can't live outside the host and
must replicate within it
- rickettsiae and chlamydia
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BACTERIAL CELL STRUCTURE &

FUNCTION

what are the 5 major groups of microorganisms? - ANSWER-1) bacteria (prokaryote)

  1. fungi (eukaryote) -> yeast & mold
  2. viruses
  3. Protozoa (eukaryote)
  4. Algae the cytoplasmic membrane is a phospholipid bilayer that .... - ANSWER-1) acts as a barrier to the environment
  5. facilitates transport in and out of the cell
  6. has extensive metabolic activity What is peptidoglycan? - ANSWER-a network of sugar polymers cross-linked by polypeptides (NAM-NAG) Which type of bacteria contain a very thick layer of peptidoglycan? - ANSWER-gram positive which type of bacteria contain an inner and an outer membrane? - ANSWER-gram negative Lipopolysaccharide (LPS) - ANSWER-Molecule that makes up the outer layer of the outer membrane of Gram-negative bacteria O-antigen - ANSWER-immune activator from LPS that is responsible for many signs of inflammatory syndrome what are the three bacterial shapes? - ANSWER-bacillus (rod) coccus (sphere) spirillus (spiral) what are 4 types of special bacteria? - ANSWER-1) naturally variant streptococci
  7. bacterial L forms
  8. bacteria that naturally lack a cell wall
  9. obligate intracellular bacteria bacterial L forms - ANSWER-temporary artificially induced variants of bacteria that lose their cell wall due to environmental factors such as abx exposure obligate intracellular bacteria - ANSWER-bacteria that can't live outside the host and must replicate within it
  • rickettsiae and chlamydia

what is the process by which bacterial replication occurs? - ANSWER-binary fision T/F: bacterial DNA is double stranded, circular, and within in a nucleoid in the cytoplasm

  • ANSWER-TRUE bacterial plasmids - ANSWER-small, circular, mobile, double stranded pieces of DNA that contain accessory genes such as virulence factors and abx resistance genes origin of replication - ANSWER-Site on DNA where replication begins (DNA opens up adjacent to the origin of replication) DnaA - ANSWER-protein that activates initiation of DNA replication in bacteria T/F: bases are added to the 3' hydroxyl end - ANSWER-TRUE leading vs lagging strand - ANSWER-leading = continuous synthesis lagging = DNA is synthesized in fragments (Okazaki fragments) where bacterial replication fork - ANSWER-bacterial DNA is replicated at TWO replication forks on either end all around the circular chromosome
  • replication is bidirectional DNA supercoiling in bacteria - ANSWER-refers to the process where bacterial DNA is twisted and coiled upon itself (usually in a negative state) to all for compact packing of the genome within the cell negative supercoils - ANSWER-circular DNA with fewer than the normal number of turns of the helix
  • allows for easier separation of the strands to allow for replication machinery to access the strands DNA gyrase (topoisomerase II) - ANSWER-relieves DNA supercoiling -> introduced negative supercoils topoisomerase I - ANSWER-single strand DNA cut topoisomerase IV - ANSWER-double DNA cut bacterial ribosome - ANSWER-70 S sedimentation (50 S and 30 S)
  • site for protein synthesis from mRNA strand what direction is mRNA synthesized? - ANSWER-5' to 3'

bacterial energy production: anaerobic - ANSWER-uses glycolysis and fermentation

  • produces acid and alcohol byproducts
  • varying degrees of oxygen tolerance among species bacterial energy production: facultative - ANSWER-will use oxygen if its present but can switch to fermentation in an anaerobic environment bacterial energy production: microaerophilic - ANSWER-grows best when O2 content is low membrane transport: facilitated diffusion - ANSWER-depends on the concentration gradient
  • membrane carrier does not require energy membrane transport: active transport - ANSWER-transports molecules against concentration gradient
  • requires energy 2 examples of active transport: primary vs secondary - ANSWER-primary: siderophores secondary: Na/glucose co-transporter is protein export from the cell easier or more difficult in a gram negative cell? - ANSWER-more difficult -> need a coupled transmembrane transporter since there are two membranes outer membrane porins - ANSWER-nonspecific transporters in gram negative envelope what is the main mechanism of abx resistance? - ANSWER-efflux proteins (MDR efflux pump) spontaneous mutations bacteria - ANSWER-occur at a low frequency but if a mutant organism has preferential growth, then it is expected to emerge as the dominant cell type (evolution) genetic exchange: transformation - ANSWER-"naked" DNA from the environment is taken up into the bacteria
  • scavagening bacteria from the environment genetic exchange: transduction - ANSWER-DNA transferred from one bacteria to another by a phage aka a virus inject their genome into the bacteria genetic exchange: conjugation - ANSWER-donor and the recipient connect and transfer DNA or plasmids via pili structures
  • may cross species lines

homologous recombination - ANSWER-Exchange of genetic information between homologous DNA molecules. site specific recombination - ANSWER-requires very little sequence homology between the recombining DNA molecules (unique sequence) Transposition (transposons) - ANSWER-Segment of DNA can jump (excision and reincorporation) from one location to another, can transfer genes from plasmid to choromosome and vice versa. classification of bacteria - ANSWER-- Genus species

  • common characteristics (shape, gram stain, growth conditions)
  • biochemical features (metabolism -> glucose, lactose)
  • chromosomal DNA (whole genome sequencing or 16S ribosome) MALDI-TOF - ANSWER-measures the masses of various components using mass spectrophotometer
  • a very precise way to identify bacteria what is the goal of abx therapy - ANSWER-to find differences in structure, replication, and physiology of the bacteria and exploit those difference to produce a selective toxicity to bacterial cells what are the 5 major targets for abx therapy - ANSWER-1) cell wall
  1. cytoplasmic membrane
  2. DNA replication
  3. folic acid metabolism
  4. protein synthesis (ribosome) what 3 classes of abx are beta-lactam drugs and target the bacterial cell wall? - ANSWER-Penicillin, cephalosporin, and carbapenem mechanism of action of penicillin G - ANSWER-Inh of transpeptidases (PBP's) by irreversible binding to enzyme active site (cell wall) -> acts as a bactericidal agent (suicide inhibitor) Biciilin CR - ANSWER-Pen G benzathine and procaine Bicillin LA - ANSWER-Pen G benzathine
  • releases the drug over a long period of time which form of penicillin G can be used for syphilis? - ANSWER-Bicillin LA (Pen G benzathine)

cephlalosporin abx mechanism of action - ANSWER-binds to PBPs and prevent them from cross-linking peptidoglycan, leading to disruption of cell wall synthesis and bacterial death. cephalosporin (3rd generation) modifications to enhance activity - ANSWER-1) hydrophilic -> able to navigate through porins

  1. stability against common B-lactamases
  2. R-1 side chain differences make it so ppl allergic to penicillin aren't to cephalosporins
  3. avoids efflux pumps
  4. more potent affinity to PBP what is unusual about Ceftriaxone's bacterial coverage? - ANSWER-since it is a 3rd generation cephlalosporin, it has greater coverage again gram negative BUT it still maintains great gam positive coverage Ceftraloline - ANSWER-anti-MRSA cephalosporin (5th generation cephalosporin) T/F: MRSA resistant to all B-lactams except ceftaroline - ANSWER-TRUE how is ceftroline active against MRSA? - ANSWER-ceftroline inhibits PBP2a (which is not normally inhibited by penicillin or other beta lactams) and S. aureus requires accessory PBP2a in addition to PBP
  • this acts as a mechanism to inhibit peptidoglycan synthesis in MRSA what bacteria have resistance to cephlalosporins like Ceftriaxone and Ceftraloline - ANSWER-AmpC producing gram negative rods (GNR) what is the major advantage with Ceftraloline? - ANSWER-active activity against MRSA meropenem - ANSWER-Carbapenem
  • one of the broadest spectrum abx (pseudomonas coverage) meropenem mechanism of action - ANSWER-nh of transpeptidases (PBPs) by irreversible binding to enzyme active site. Carbapenems are bactericidal agents (cell wall) T/F: Stenotrophomonas maltophilia is resistant to meropenem? - ANSWER-TRUE T/F: Carbapenems 1st choice for ESBL producing enterobacterales - ANSWER-TRUE T/F: Many hospitals attempt to restrict carbapenem use bc overuse can cause resistance problems in future - ANSWER-TRUE metronidazole - ANSWER-nitroimidazole

metronidazole mechanism of action - ANSWER-reduced by pyruvate-ferredoxin oxidoreductase leading to a reactive radical that will interact with DNA leading to strand destabilization and breakage

  • DNA synthesis is the target T/F: Metronidazole is an excellent drug against anaerobic bacteria, with 99% of bacteroides spp. being susceptible - ANSWER-TRUE (Metronidazole has anaerobic coverage) which abx has a rare neuropathy SE when the dose exceeds 42 g - ANSWER- metronidazole which abx can cause an Antabuse rxn and therefore alcohol should be avoided when on this medication - ANSWER-metronidazole isoniazid (INH) - ANSWER-isonicotinic acid hydrazine isoniazid (INH) mechanism of action - ANSWER-activated by catalase-peroxidase (KatG) to then inhibit fatty acid synthase II
  • mycolic acid synthesis is the target which abx is 1st line antibiotic for tx of Mycobacterium tuberculosis. - ANSWER- isoniazid (INH) -> RIPE combo (full tx 6 mo - 2 years) T/F: INH one of most important drugs known for causing drug-induced liver injury - ANSWER-TRUE -> age (older patients), dose, PMH, genetics, alcohol play a role in severity slow acetylators (NAT2) are more likely to get neurotoxicity/neuropathy with INH - ANSWER-TRUE -> leads to increased levels rifampin - ANSWER-rifamycin rifampin mechanism of action - ANSWER-binds to DNA-dependent RNA polymerase in prokaryotes
  • RNA synthesis is the target T/F: the most important problem with rifampin is related to induction of metabolism and resulting in drug interactions - ANSWER-TRUE -> there are alot of DDIs T/F: rifampin can also cause hepatotoxicity - ANSWER-TRUE -> but to a much lesser extent than INH what is a side effect of rifampin? - ANSWER-Pink-red color of urine and sweat