BIOD 171 MODULE 3 CERTIFICATION
EXAMINATION 2026 COMPREHENSIVE STUDY
SHEET
◉ Incubator temperature for microbial growth. Answer: 1.
Maintaining a temperature of 37°C is optimal for promoting growth
and is the most commonly used
2. Other organisms such as yeast preferentially grow at 30°C
3. Researchers may set incubators at 25°C to restrict pathogenic
growth, because pathogenic strains of bacteria tend to grow faster
than non-pathogenic strains at 37°C
◉ Analyzing unknown causative agent. Answer: The first step is to
expand any potential microbial populations.
The easiest way to do this is to inoculate a simple growth media with
the collected sample or streak it onto an agar plate. The culture (or
plate) is then grown at 37°C to encourage microbial growth.
Once a bacterial population has been expanded, samples can be
taken and re-streaked onto selective and/or differential media for
further analysis
◉ Universal precautions. Answer: All handlers must treat all
samples, known or unknown, as potentially hazardous (or
pathogenic) materials.
