Biotechnology Study material for UPSC students, Study notes of Biotechnology

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CELL:-
The cell is the structural and functional unit of life, which means that the structure of our
body is made up of cells, and all the functions we perform are done by the cell.
Cells, as the fundamental units of life, possess a complex structure that allows them to
carry out various functions necessary for the existence and functioning of living
organisms.
Cells are capable of independent existence.
Living beings can be:
o Unicellular organisms Made up of a single cell. Example: Amoeba, bacteria, fungi,
paramecium, etc
o Multicellular organisms Composed of multiple cells. Example: Humans, animals,
plants
Cells come in various shapes and sizes, such as muscle cells, neuron cells, sperm cells, and
egg cells. Their combination forms an organism.
Characteristics of the Cell:
Reproduction: Cells play a role in both sexual and asexual modes of reproduction.
Cell structure: It includes topics such as the division of labour.
Metabolism: Metabolism covers digestion, excretion, respiration, and blood circulation.
Adaptation: There are two types of adaptation: short-term adaptation and long-term
adaptation.
Discovery of the Cell:
Robert Hooke (1665): The discovery of the cell is credited to Robert Hooke, an English
scientist, who first observed cells in 1665.
o Hooke was examining a thin slice of cork under a microscope.
o The cork appeared to be made up of tiny, box-like structures, which he called "cells"
because they resembled the small rooms (cells) that monks lived in.
Anton van Leeuwenhoek (1674): Anton van Leeuwenhoek, a Dutch scientist, made
significant improvements to the microscope.
o He was the first to observe live cells, including bacteria, red blood cells, and sperm
cells. He discovered free-living cells in pond water.
Robert Brown (1831): In 1831, while examining orchid cells under a microscope,
Robert Brown observed a small, round structure inside the cell. He identified this
structure as the nucleus.
o He was the first to describe the nucleus and recognize its importance within the cell.
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❖ CELL:-

The cell is the structural and functional unit of life, which means that the structure of our body is made up of cells, and all the functions we perform are done by the cell.

  • Cells, as the fundamental units of life, possess a complex structure that allows them to carry out various functions necessary for the existence and functioning of living organisms.
  • Cells are capable of independent existence.
  • Living beings can be: o Unicellular organisms – Made up of a single cell. Example: Amoeba, bacteria, fungi, paramecium, etc o Multicellular organisms – Composed of multiple cells. Example: Humans, animals, plants
  • Cells come in various shapes and sizes, such as muscle cells, neuron cells, sperm cells, and egg cells. Their combination forms an organism. Characteristics of the Cell:
  • Reproduction: Cells play a role in both sexual and asexual modes of reproduction.
  • Cell structure: It includes topics such as the division of labour.
  • Metabolism: Metabolism covers digestion, excretion, respiration, and blood circulation.
  • Adaptation: There are two types of adaptation: short-term adaptation and long-term adaptation. Discovery of the Cell:
  • Robert Hooke (1665): The discovery of the cell is credited to Robert Hooke, an English scientist, who first observed cells in 1665. o Hooke was examining a thin slice of cork under a microscope. o The cork appeared to be made up of tiny, box-like structures, which he called "cells" because they resembled the small rooms (cells) that monks lived in.
  • Anton van Leeuwenhoek (1674): Anton van Leeuwenhoek, a Dutch scientist, made significant improvements to the microscope. o He was the first to observe live cells, including bacteria, red blood cells, and sperm cells. He discovered free-living cells in pond water.
  • Robert Brown (1831): In 1831, while examining orchid cells under a microscope, Robert Brown observed a small, round structure inside the cell. He identified this structure as the nucleus. o He was the first to describe the nucleus and recognize its importance within the cell.
  • Jan Evangelista Purkinje: In the context of Purkinje cells in the cerebellum, Purkinje's work also extended to the study of the protoplasm in neurons. o While Purkinje specifically coined the term protoplasm, which includes cytoplasm and nucleus. o His research on cells contributed to the general understanding of protoplasmic structures in both muscle and nerve tissue.
  • The Cell Theory (1830s): o Matthias Schleiden (1838): German botanist Matthias Schleiden proposed that all plants are made up of cells. ▪ His work laid the foundation for the idea that cells are the fundamental units of life in plants. o Theodor Schwann (1839): German zoologist Theodor Schwann extended this idea to animals, proposing that all living organisms, both plants and animals, are made of cells. ▪ The single cell is known as a zygote, and the human body contains trillions of cells. ▪ This led to the formulation of the first part of the Cell Theory, which states that all living things are composed of cells. o Rudolf Virchow (1855): German physician Rudolf Virchow added the final piece to the Cell Theory by stating that all cells are derived from pre-existing cells. ▪ This completed the modern understanding that cells are the basic structural and functional units of life.
  • The electronic microscope was developed in 1940 Various Components of the Cell:
  • Cell Membrane (Plasma Membrane): It is a selectively permeable lipid bilayer made up of phospholipids, proteins, and carbohydrates.
  • Cytoplasm: It is a gel-like substance filling the cell's interior.
  • Nucleus: It is a Membrane-bound organelle containing genetic material (DNA).
  • Cell wall: The cell wall is a rigid, protective outer layer found only in plant cells. It provides structural support and protection and helps maintain the shape of the cell. Cell structure (Organelles):
  • Outer cover: o Cell wall (Only in plant cells) o Cell membrane or plasma membrane
  • Cytoplasm: o Mitochondria

Semi-Permeable: Allows only certain substances to pass through. Selectively Permeable: Regulates and controls which substances can enter and exit. Non-Permeable: Does not allow any substances to pass through. ❖ Cytoplasm

  • It is a semi-fluid substance inside the cell membrane.
  • It acts as a storage site for various organelles.
  • It is present between the nucleus and the plasma membrane. Mitochondria (Powerhouse of the Cell)
  • Function: It is called the "powerhouse of the cell" as it produces energy by breaking down food through the oxidation of glucose in the presence of oxygen (i.e. respiration of the cell).
  • Process: During cellular respiration, a glucose molecule with oxygen is gradually broken down into carbon dioxide and water. It releases energy as well, which is in the form of ATP. o ATP (Adenosine Triphosphate) is the energy currency.
  • Structure: It is a double-layered organelle. o The mitochondrial matrix is the viscous fluid that contains mitochondrial DNA. It has a dual membrane structure and has its own DNA. Hence, mitochondria are semi- autonomous organelles. o Cristae: Cristae are intricate structures within mitochondria that play a role in cellular respiration. o Mitochondrial DNA (mtDNA) gives it partial independence. ▪ Matrilineal Inheritance: Children get mitochondrial DNA from their mother (but not their father). In other words, a child inherits mitochondrial disorders entirely from the mother's side and not from the father. o A semi-autonomous organelle is one that can divide independently during cell division and perform essential functions, such as energy production, due to the presence of mitochondrial DNA (mtDNA). Plastids
  • It is available in plants. Plastid has a double membrane structure and has its own plastid DNA and therefore it is a semi-autonomous organelle.
  • Plastids also possess DNA and ribosomes, which are responsible for protein synthesis.
  • It is of the following types:

o Chloroplast: They are colorful (Pigments). It will give a green colour when Chlorophyll is present. o Chromoplast: It will give an orange colour when Carotene is present. It will give a yellow colour when Xanthophylls is present. All these also help in Photosynthesis o Leucoplast: It is colourless/transparent because no pigment is present. Its function is to store food for plants. E.g. starch.

  • Structure: o It is also a double-membrane structure. Its internal structure includes grana and thylakoids. The fluid present within it is known as stroma. o It also contains its own DNA. Since it carries its own DNA, this organelle is considered semi-autonomous. Note: Manipulating plastid function can improve crop yield, nutritional content, and environmental resilience. Hence, it is crucial for agrobiotechnology. Endoplasmic Reticulum and Ribosomes
  • Ribosomes: Ribosomes help in synthesising proteins. Ribosomes are made of proteins and rRNA.
  • Endoplasmic Reticulum (ER): Endoplasmic Reticulum is a single-layered organelle o It is like a maze (or tube or sac-like structure) surrounding the nucleus that connects with the nuclear membrane and runs throughout the cytoplasm. o The rough endoplasmic reticulum (rough ER) gets its name from the bumpy ribosomes attached to its surface. o Smooth Endoplasmic Reticulum (Smooth ER) plays a key role in the synthesis of lipids (fatty compounds), phospholipids, and steroids (Eg; testosterone, estrogen, progesterone). It helps in the detoxification of drugs and poisons in liver cells. Golgi Bodies:
  • Golgi bodies originate from the Endoplasmic Reticulum (ER).
  • Its functions include the packing and transportation of protein. It can modify proteins into different enzymes (e.g. lysosomes).
  • The nucleolus is also located within the nucleus.
  • The nucleolus was first described in the early 1830s as a "nucleus within the nucleus," with the name "nucleolus" coined by the German physiologist Gabriel Gustav Valentin.
  • In humans, chromosomes become visible during cell division. Humans have 23 pairs (46 total) of chromosomes.
  • Nucleolus helps in the production of ribosomes.
  • Cells can not be seen with open eyes.
  • The largest cell is of the Ostrich’s egg.
  • Functions of a Nucleus: It contains genetic material. o Chromosomes: The nucleus contains thread-like structures called chromosomes. Chromosomes are attached to the histones that provide structural support for a chromosome. It is only visible during cell division. o A complex genetic material found inside the nucleus is called chromatin, also referred to as the chromatin network or chromatin complex. o Histone Protein: It is a non-genetic material and is common among humans. They provide structural support or stability of DNA. Histone proteins help in packaging DNA. Deoxyribonucleic Acid (DNA):
  • DNA is in a double helical structure and is the genetic material of the cell.
  • This is the genetic material, and all the genetic information is written on it. Every human has 99.9 percent of the same DNA structure. The rest 0.01 percent differ. This difference makes every human different from one another. Gene:
  • Gene is the functional segment/unit of DNA. This tells us that not all parts of DNA are functional.
  • Polygenic Inheritance: Normally, one gene determines one trait/character/phenotype. However, sometimes a single character can be determined by multiple sets of genes. This is known as the polygenic effect. Example: Colour of our skin.
  • There are approximately 30,000 genes in one cell.
  • It carries instructions for making proteins or RNA.
  • Genes are located on chromosomes in the cell nucleus.
  • Genes are inherited from parents and passed to offspring. Genome:
  • It is the complete set of an organism’s genetic material.
  • It includes all the genes and non-coding DNA.
  • The genome is found in the cell’s nucleus.
  • It is made up of DNA in humans and RNA in some viruses.
  • The genome carries all the information needed for growth and development.
  • It is inherited from both parents and passed to offspring.
  • The genome is organized into chromosomes.
  • Variations in the genome contribute to individual differences. Types of Cells:
  • Prokaryotic Cells: Prokaryotic Cells are the simplest type of cells. They do not have a nucleus or other organelles. They are found in bacteria and archaea.
  • Eukaryotic Cells: Eukaryotic Cells are more complex than prokaryotic cells. They have a nucleus and other organelles, such as mitochondria and chloroplasts. Eukaryotic cells are found in plants, animals, fungi, and protists. Prokaryotic Cells have the Following Features:
  • They do not have a nucleus.
  • Their DNA is not enclosed in a membrane.
  • They have a single circular chromosome.
  • They have ribosomes, but they are not bound to the endoplasmic reticulum.
  • They do not have mitochondria or chloroplasts.
  • Examples: Bacterial cells (Amoeba, Paramecium) Extra Edge The Structure of a Bacterial Cell: ✓ Cell membrane: The cell membrane is a thin, flexible layer that surrounds the cell. It controls what enters and leaves the cell. ✓ Cytoplasm: The cytoplasm is the jelly-like substance inside the cell. It contains all of the cell’s organelles, as well as the cell’s DNA. ✓ Nucleoid: The nucleoid is the region of the cytoplasm where the cell’s DNA is located. It is not enclosed by a membrane, like the nucleus of a eukaryotic cell. ✓ Ribosomes: Ribosomes are tiny organelles that make proteins. They are found in the cytoplasm and in the endoplasmic reticulum. ✓ Plasmid: A plasmid is a small, circular piece of DNA that is not part of the cell’s main chromosome. Plasmids can carry genes that code for different traits, such as antibiotic resistance.
  • M: This is the mitotic phase, where the cell divides into two daughter cells. During this phase, the chromosomes condense, the nuclear membrane breaks down, and the mitotic spindle forms. The mitotic spindle is a structure that helps to separate the chromosomes during mitosis. The chromosomes then separate and move to opposite poles of the cell, and the cell divides into two daughter cells. Cell Division:
  • Mitosis: o It is a type of cell division that results in two identical daughter cells. o The number of chromosomes remains the same in the daughter cells as in the parent cell. o It is used for the growth and repair of the body.
  • Meiosis: o It is a type of cell division that results in four daughter cells. o The number of chromosomes is reduced by half in the daughter cells compared to the parent cell. o It is used for the production of gametes (sex cells). Mitosis Division:
  • Nuclear division is the process by which the nucleus of the cell divides into two daughter nuclei. This happens in the m phase of mitosis, which is the stage where the chromosomes are duplicated and the cell prepares to divide.
  • The chromosomes are duplicated in the first stage of mitosis, called prophase. In this stage, the chromosomes condense and become visible. The nuclear membrane breaks down and the nucleolus disappears.
  • In the second stage, called the metaphase, the chromosomes line up in the middle of the cell. In this stage, the chromosomes line up in the middle of the cell.
  • In the third stage, called the anaphase, the chromosomes separate and move to opposite poles of the cell. In this stage, the chromosomes are separated and move to opposite poles of the cell.
  • In the fourth and final stage, called the telophase, the nuclear envelope reforms around each set of chromosomes, and the cell divides into two new cells. In this stage, the nuclear membrane reforms and the nucleolus reappears. The cell then divides into two daughter cells. Mitosis vs Meiosis Mitosis Meiosis

Provides a 'repair and maintenance' service to old and damaged body cells. It plays a crucial role in germ cell production. Mitosis occurs in somatic cells; this means that it takes place in all types of cells that are not involved in the production of gametes. Meiosis contains two separate cell divisions, meaning that one parent cell can produce four gametes (eggs in females, sperm in males). In each round of division, cells go through four stages: prophase, metaphase, anaphase, and telophase. Mitosis is called equational division because the number of chromosomes in daughter cells remains equal to parent cells. Meiosis is called reductional division because the number of chromosomes in daughter cells is reduced to half that of the parent cells. Mitosis produces diploid cells Meiosis produces a haploid cell Mitosis is the type of cell division by which a single cell divides. Two divisions, meiosis I and meiosis II, are required to produce gametes The Process of Cell Division in a Human Cell:

  • Human cells have 46 chromosomes, which are made up of DNA. Meiosis is a type of cell division that produces gametes, or sex cells. In meiosis, the number of chromosomes is reduced by half, so each gamete has 23 chromosomes. This is because a human cell has 23 pairs of chromosomes, for a total of 46 chromosomes. In meiosis, each chromosome pair separates, so each gamete only gets one chromosome from each pair.
  • Male cells produce sperm cells, which are the male gametes. Female cells produce egg cells, which are the female gametes. When a sperm cell fertilizes an egg cell, the two gametes fuse together to form a zygote. The zygote has 46 chromosomes, the same number of chromosomes as a human cell. This is because the sperm cell and egg cell each contributed 23 chromosomes to the zygote.
  • Cell division is used to repair cells that have been damaged or injured. For example, if a person gets a cut, the cells in the cut area will divide to repair the damage. This process is called a Rep cell. Somatic Cells and Gamete Cells: Two Main Types of Human Cells Somatic Cells
    • Somatic cells make up all the tissues and organs in the human body.
    • Each somatic cell contains 46 chromosomes, arranged in 23 pairs.
    • These chromosomes are made up of DNA, the genetic material that determines a person's traits.

o Phosphate (PO4): Each nitrogenous base in a nucleotide is attached to a sugar molecule, which is attached to one or more phosphate groups. ▪ A nucleotide is the basic building block of nucleic acids (RNA and DNA). o Nitrogenous Bases: The information in DNA is stored as a code made up of four chemical bases. In other words, each nucleotide in DNA contains one of four possible nitrogenous bases: adenine (A), guanine (G), cytosine (C), and thymine (T). ▪ Purines and Pyrimidines: Adenine and guanine are classified as purines. Cytosine, thymine, and uracil are classified as pyrimidines. ▪ Sequencing: The order, or sequence, of these bases determines the information available for building and maintaining an organism, similar to the way in which letters of the alphabet appear in a certain order to form words and sentences. ▪ Pairing: DNA bases pair up with each other ✓ Adenine (A) - Double hydrogen bond with Thymine ✓ Thymine (T) - Double hydrogen bond with Adenine ✓ Guanine (G) - Triple hydrogen bond with Cytosine ✓ Cytosine (C) - Triple hydrogen bond with Guanine ▪ This is a complementary sequence. ▪ Uracil (U): RNA (Ribonucleic Acid) nucleotides may also bear adenine, guanine and cytosine bases, but instead of thymine, they have another pyrimidine base called uracil (U). RNA Nitrogen Bases: ✓ Adenine (A) - Bond with Uracil ✓ Uracil (U) - Bond with Adenine ✓ Guanine (G) - Bond with Cytosine ✓ Cytosine (C) - Bond with Guanine Genetic Language:

  • Genetic Language of DNA: In DNA, the genetic code is read in triplets, also known as codons, where each three-base sequence (like CAG or TTA or TAC, etc) codes for a specific amino acid, forming units/segments of DNA that ultimately determine protein synthesis. o There are 64 possible three-base combinations in the genetic code. o The sequence of these codons encodes genetic information, much like sentences form meaningful words.
  • Just like the binary language (computer language) where information is represented using only two symbols, or binary digits of 1 and 0 code arranged in ways that the computer can read, understand, and act upon, we have genetic language based on four symbols namely A, T, C, G. Central Dogma of Cellular Biology It is the process by which genetic information flows only in one direction that is from DNA, to RNA, to protein.
  • RNAs is like photocopy of the genetic information carried by DNA. o RNA polymerase is an enzyme that catalyzes the synthesis of RNA from a DNA template during the process of transcription. o There is a special type of RNA known as mRNA (messenger RNA), which transfers genetic information from DNA to ribosomes. This process is known as transcription. o Once these mRNAs reach ribosomes, genetic information is transferred by tRNA (transfer RNA). This tRNA carries amino acids. o Thus a long chain of amino acids is formed, which is then restructured to form protein. This process of making a protein is known as Translation.
  • Transcription: The DNA is copied to make messenger RNA (mRNA). This step is called transcription because it involves rewriting or transcribing, the DNA sequence in a similar RNA
  • Translation: The process by which mRNA directs protein synthesis with the assistance of tRNA is called translation.
  • Exception to Central Dogma: Typically, in the process of transcription, DNA is transcribed into mRNA. However, in reverse transcription, a virus (like a Retrovirus) is able to use mRNA to convert into double-stranded DNA
  • Replication: DNA replication is a process by which a double-stranded DNA molecule is copied into two, identical DNA molecules. Reverse Transcription
  • Entry into Host Cell: A retrovirus (e.g., HIV) has a spike protein on its surface that functions like a "key" to enter the host cell. This key binds to specific receptors on the surface of the host cell (bacteria in this case). Uncoating and Release of RNA: Once inside the host, the retrovirus uncoats itself, releasing its genetic material (which is in the form of single-stranded RNA) into the host cell's cytoplasm.

Collection Study DNA Genome Genomic mRNA Transription Transcriptomics Protein Proteome Proteomics Metabolism Metabolome Metabolomics DNA Functions:

  • DNA undergoes replication during cell division to ensure genetic continuity. A collection of all DNA in an organism is termed the genome. The study of genomes is called genomics.
  • Transcription (DNA to RNA): o DNA is transcribed into mRNA. o Reverse transcription (RNA to DNA) is also possible.
  • RNA and Transcriptomics: o All RNA molecules formed from transcription constitute the transcriptome. The study of transcriptomes is known as transcriptomics.
  • Translation (RNA to Protein): o mRNA is translated into proteins. All proteins in a cell are referred to as the proteome. The study of the proteome is termed proteomics. Structure of RNA:
  • RNA is a single-stranded molecule made up of nucleotides. Nucleotides are the basic building blocks of RNA, and they each contain a sugar group, a phosphate group, and a nitrogenous base.
  • The four types of nitrogenous bases in RNA are adenine (A), guanine (G), cytosine (C), and uracil (U). o RNA Nitrogen Bases: ▪ Adenine (A) - Bond with Uracil ▪ Uracil (U) - Bond with Adenine ▪ Guanine (G) - Bond with Cytosine ▪ Cytosine (C) - Bond with Guanine
  • The nucleotides in RNA are linked together by phosphodiester bonds. These bonds form a sugar-phosphate backbone, which is the same as the sugar-phosphate backbone in DNA.
  • The nitrogenous bases in RNA can pair with each other to form hydrogen bonds. Adenine pairs with uracil, and guanine pairs with cytosine. This pairing of bases is known as complementary base pairing.
  • The structure of RNA is essential for its function. RNA plays many roles in the cell, including:

o Transcription: RNA is involved in the process of transcription, which is the process of copying DNA into RNA. o Translation: RNA is involved in the process of translation, which is the process of converting RNA into proteins. o Regulation: RNA is involved in the regulation of gene expression. Extra Edge: Double-Stranded RNA: Although RNA is a single-stranded molecule, researchers soon discovered that it can form double-stranded structures In 1956, Alexander Rich and David Davies, both working at the National Institutes of Health, discovered that single strands of RNA can "hybridise," sticking together to form a double-stranded molecule. Location of DNA in Cell Organelles:

  • Nucleus: Primary location of DNA in eukaryotic cells.
  • Mitochondria: Contains mitochondrial DNA (mtDNA) — maternally inherited; makes mitochondria semi-autonomous.
  • Plastids (in plants): Contains plastid DNA (ptDNA) — makes plastids semi-autonomous as well. Genetic Mutation:
  • It is the change in DNA sequence within the genome of an organism.
  • Reasons: It mainly happens due to o Errors during DNA replication. Example: Sickle Cell Anemia. o Due to environmental factors. Example: Genetic changes in people born in the highly radioactive region of Hiroshima and Nagasaki.
  • Impact: Genetic mutation leads to changes in the phenotypic changes (physical characteristics) of an organism or affects its ability to survive and reproduce. It plays a crucial role in evolution and genetic diversity. DNA-Related Terms
  • Single Nucleotide Polymorphism (SNPs): A DNA sequence variation occurs when a single nucleotide base pair (A, T, G, or C) differs between individuals of the same species. o They can act as biological markers, helping scientists identify genes associated with diseases. Example: Sickle cell anaemia. o SNP doesn't always symbolise some kind of disease. There are thousands of SNPs in every species yet they can feel normal and free from disease. o SNPs are generally not used in forensic studies as many SNPs are common among various members of a species.

o Application: To identify similar species (e.g. Leopard & Jaguar or Rabbit & Hare) ▪ It is used to check food adulteration ▪ It is often used for fish species identification. ▪ It is also used for biodiversity research and conservation

  • Aerial Metagenomics: o Meaning: It is the study of microorganisms through the direct extraction and analysis of DNA from their collective environment. o Understanding Through an Example: A dog in a specific area is falling ill after drinking from a local pond. To determine the cause of its sickness, we can use metagenomics to analyze both the pond water and the dog's gut/excretion samples. ▪ Database 1 (Pond Water): We collect a sample of the pond water to identify the microorganisms present (bacteria, viruses, protozoa, etc.). ▪ Database 2 (Dog's Excretion/Gut): We then collect samples from the dog's feces sample to identify the microorganisms that may be contributing to its illness. ▪ Comparative Analysis: By matching genetic sequences from both databases, we can identify any common microorganisms or pathogens that might explain the dog's illness. ▪ In Aerial Metagenomics, the sample would be collected from the air rather than the pond. ❖ VIRUS :-
  • The virus is a connecting link between living and nonliving organisms. It is like a 'living dead'. o Living: A virus has the ability to reproduce. o Non-Living: A Virus is considered nonliving because it does not perform any kind of metabolic activity. o The virus is living only when it enters a living cell. When the virus is outside any living cell, it is non-living.
  • Working: A virus looks for a host (living cell) and, when found, can completely take over it or hijack its metabolism. It is able to do this due to the genetic material found inside it. o Virus Reproduction: It starts producing a protein of its own kind and multiplies in number by destroying the cell. This is called virus reproduction.
  • Structure: It is made up of DNA/RNA, which is a genetic material, and is protected/enveloped by a protein shell called a capsid.
  • In all microorganisms (like fungi, bacteria, etc.), viruses can never be synthesized in the lab because they lack any kind of metabolism. Types of Viruses
  • Based on Genetic Material: o DNA-Based Virus (Adenovirus is DNA-based) ▪ Examples: Pox virus, Hepatitis B Virus. o RNA based virus (Retrovirus) ▪ Examples: HIV, Corona, Ebola, Mumps, Measles, Zika, Dengue, Nipah, Hepatitis A,C,D,E.
  • Based on Infection Target: o Animal-Based Viruses: Infect animals. ▪ Examples: HIV, Coronavirus, Measles, Mumps, Hepatitis o Plant-Based Viruses: Infect plants. ▪ Examples: Tobacco/Cauliflower Mosaic Virus, Potato Leaf Roll Virus o Bacteriophages: Infect bacteria. o Mycophages: Infect fungi.
  • Note: In this living world, DNA and RNA both are present as genetic Materials but RNA as genetic material is only present in Retrovirus. o RNA-based genetic material was the first Genetic Material that appeared on earth. Human Genome Project: It was started in the USA (1990–2003) and was supported by many developing countries.
  • Objective: The entire human genome was studied for the first time in this project. A co mplete sequence of nitrogen bases (base pairs) in the genetic material was determined. Also known as genome sequencing or gene mapping.
  • Outcome of the Human Genome Project: o Identified 3.3 billion nitrogen base pairs. o Discovered approximately 30,000 genes. o Found that more than 50% of the DNA sequence consists of junk DNA. o Determined that in any particular cell, only 2% of genes are switched on (active) and involved in protein synthesis. o Revealed that 99.9% of genetic material is the same in all human beings, with the differences between individuals due to only 0.1% genetic dissimilarity. ❖ IMMUNE SYSTEM :- Immune System