MagHelix™ Zebrafish Screening: Comprehensive Solution for Drug Discovery & Toxicology, Slides of Biology

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Zebrafish Screening Platform

Zebrafish (Danio rerio) possesses the physiology complexity of

mammalian animals and is one of the most common vertebrate model

organisms. Creative Biostructure establishes the MagHelix™ Zebrafish

Screening Platform for medical research. Zebrafish is an excellent

choice to model various diseases and screen novel drugs for medical

and therapeutic research. Zebrafish larvae have been used

increasingly within the last decade to assess the effects of chemical

compounds.

Advantages of the Zebrafish as Animal Model

Small size makes their embryos and larvae dispensed in microtiter plates and ease to

manipulate.

Zebrafish has high fecundity so it is easy to obtain large sample numbers.

Embryo and larvae are transparent to facilitate noninvasive imaging, enabling massive

phenotype-based screening.

High genetic homology to humans (> 85%) as well as significant similarities in

organogenesis and functional mechanisms.

The occurrence of numerous pathological processes is similar to those of many human

diseases.

There are few ethical issues associated with the use of larvae up to the feeding stage.

They have permeability to small molecules which allows drug to administrate by

immersion.

Small amount of sample is enough.

Short period for experiment.

Applications of the zebrafish as an animal model include both identification of endpoints of

toxicity and elucidation of mechanisms of toxicity.

There are four classes of zebrafish chemical screening assays:

Morphological screens (to screen chemical genetics and toxicology)

Behavioral screens (ex. using swimming kinetics to quantify the effects

of single compounds)

Fixed time-point/labeling assays (to obtain information on a cellular or

tissue level)

Fluorescence assays (reporter-based quantifications)

Creative Biostructure welcomes global academic institutions, biotechnology, and

pharmaceutical industries to utilize our MagHelix™ Zebrafish Screening Platform

to evaluate the activity and toxicity of compounds for drug development.

References

Taylor K L.; et al. Small molecule screening in zebrafish: an in vivo

approach to identifying new chemical tools and drug leads. Cell

Communication and Signaling. 2010, 8(1): 11.

Mathias J R.; et al. Advances in zebrafish chemical screening

technologies. Future Medicinal Chemistry. 2012, 4(14): 1811-1822.

Kitambi S S.; et al. Small molecule screening platform for assessment of

cardiovascular toxicity on adult zebrafish heart. BMC Physiology. 2012,