Biochemistry Study Notes, Study Guides, Projects, Research of Biochemistry

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2025/2026

Uploaded on 04/20/2026

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Practice Exam questions
Instructions: This is designed to help you prepare for your upcoming test. Answer all
questions. Show all work for calculations. Assume all multiple-choice questions have one
best answer unless otherwise stated.
Multiple Choice (10 questions)
1. Which of the following units indicates solution concentration by itself?
a) mL
b) M
c) g
d) mol
2. How many micrograms are in 0.5 mg?
a) 5 µg
b) 50 µg
c) 500 µg
d) 5000 µg
3. A 2 M solution is equivalent to which of the following?
a) 200 mM
b) 0.002 mM
c) 2000 mM
d) 20 mM
4. If 1.5 mol of solute is dissolved in 0.75 L, what is the molarity?
a) 0.5 M
b) 1 M
c) 2 M
d) 3 M
5. How many moles are present in 250 mL of a 0.5 M NaCl solution?
a) 0.125 mol
b) 2 mol
c) 125 mol
d) 0.02 mol
6. Which concentration is equivalent to 50 µM?
a) 0.05 mM
b) 5 mM
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Practice Exam questions Instructions: This is designed to help you prepare for your upcoming test. Answer all questions. Show all work for calculations. Assume all multiple-choice questions have one best answer unless otherwise stated. Multiple Choice (10 questions)

  1. Which of the following units indicates solution concentration by itself? a) mL b) M c) g d) mol
  2. How many micrograms are in 0.5 mg? a) 5 μg b) 50 μg c) 500 μg d) 5000 μg
  3. A 2 M solution is equivalent to which of the following? a) 200 mM b) 0.002 mM c) 2000 mM d) 20 mM
  4. If 1.5 mol of solute is dissolved in 0.75 L, what is the molarity? a) 0.5 M b) 1 M c) 2 M d) 3 M
  5. How many moles are present in 250 mL of a 0.5 M NaCl solution? a) 0.125 mol b) 2 mol c) 125 mol d) 0.02 mol
  6. Which concentration is equivalent to 50 μM? a) 0.05 mM b) 5 mM

c) 0.0005 mM d) 50 mM

  1. What volume of a 10 mg/mL stock is needed to make 100 μL of 1 mg/mL solution? a) 1 μL b) 10 μL c) 50 μL d) 90 μL
  2. How much Tris (MW = 121 g/mol) is needed to make 1 L of 50 mM solution? a) 6.05 g b) 0.605 g c) 60.5 g d) 12.1 g
  3. Which reagent is commonly used to induce protein expression in E. coli? a) Agar b) IPTG c) NaCl d) Glucose Short Answer Briefly explain the general steps involved in preparing a buffered solution from dry powder. A 25 μL reaction contains 2 μL primer stock for a final concentration of 1 μM. What is the stock concentration?

Explain what the non-pipetting hand is doing when setting up a small volume sample using good pipetting technique? A student describes a balanced rotor has having the tubes in the correct location. Is the student’s description accurate, incomplete, wrong, or a combination of both. Explain Explain Hydrophobic interaction chromatography. Did we use it for our project? If so, explain. Explain the role that IPTG plays in the work you have conducted so far in this semester’s project. What was the purpose of placing the conical tubes containing LB-agar into the water bath set at 50-55oC? If this incubation step was skipped, how would results of “streaking for colonies” be affected.