Cryo-EM: A Powerful Tool for Structural Research of Protein-Ligand Complexes, Slides of Biology

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Protein-Ligand

Complex Structure

Determination

All living organisms are dependent on two essential biopolymers for

their biological functions: nucleic acids and proteins. Nucleic acids (refer

to DNA and RNA) are competent to encode and store genetic

information in the nucleus of every living organism on Earth. Like the

nucleic acids, proteins also are essential parts of organisms and

participate in virtually every process within cells. Proteins have the

ability to bind other molecules specifically and tightly, such as nucleic

acids, other proteins as well as small-molecule substrates and so on.

The interaction of biomacromolecules is the fundamental basis of

cellular function. Thus it is very important for understanding the

biological functions of proteins that studying the interaction of proteins

and ligands.

With the new generation of electron detectors and advanced image

processing, Cryo-EM has recently undergone a quantum leap

progression in resolution and improved its structure determination by

single-particle analysis. Without crystallization and only needing a small

amount of samples in solution, Cryo-EM can be used to study the

dynamic progress of functional complexes, which are often unstable,

flexible, transient in their native environments. Due to it can provide a

high-resolution tomography and three-dimensional (3D) reconstruction

in near-atomic level, Cryo-EM has become a powerful tool for the

structural research of biological macromolecular complexes, such as

protein-DNA/RNA complexes and protein-ligands, etc.

At Creative Biostructure , we have a wealth of experience in studying

the structures of biological macromolecules and its complexes by

utilizing Cryo-EM, such as the study of proteins, DNA

samples, ribosomes, antigen-antibody complex, and so on. In fact, there

has been reported many Cryo-EM structures of biological

macromolecules and its complexes. For example, eukaryotic translation

complexes provides an abundance of biological insights for

understanding the biological progress of translation.

Creative Biostructure promises to work closely with our customers to

provide excellent services of structural studies with unique biological

insights.

Excerpt from:

https://www.creative-biostructure.com/Cryo-EM-for-Protein-

Ligands-Complex-602.htm