Cryo-EM Analysis of Bacteriophages: High-Res Structures for Antibacterial Research, Slides of Biology

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Bacteriophage

Structure

Determination

It is crucial that understanding the biological functions of macromolecules by characterizing the molecular structure. As the most effective method to obtain atomic models of proteins and nucleic acids in the 20th century, X-ray crystallography has been fundamental in the development of many scientific fields. But many samples have been proved that it is difficult to crystallize (for example membrane proteins). In addition, the biological macromolecular complexes sometimes cannot be produced in sufficient quantities of crystallization. Cryo-Electron Microscopy (Cryo-EM) has become a potential alternative to X-ray crystallography that has quickly gained popularity in structural biology.

Due to Cryo-EM does not require dehydration of the samples and can produce near atomic-level model, it has been proved that Cryo-EM has become a most convenient and advanced tool for obtaining high- resolution images of bacteriophages. There have many structures of phages obtained by Cryo-EM. For example, it had been reported that the atomic structure of bacteriophage T4 baseplate in its pre- and post- host attachment states obtained by Cryo-EM. Bacteriophage can use the tail to break through the cell surface and get into the interior of cells. With the high-resolution structure of bacteriophage, it helps to explain the events that lead to sheath contraction in atomic detail and understand the mechanism of bacteriophage infects bacteria.

At Creative Biostructure, we have the most advanced high-resolution electron microscopy equipment and a direct electron detector. Our services include the preparation and purification of bacteriophages, image processing of Cryo-EM, and finally we provide our customer with the three-dimensional (3D) reconstruction and near atomic model of bacteriophages. The ultrastructure of bacteriophage can eventually gain an insight into antibacterial research.

Creative Biostructure promises to work closely with our customers to provide excellent Cryo-EM strategies for the bacteriophages of your interest.

Excerpt from: https://www.creative-biostructure.com/Cryo-EM-for- Bacteriophages-600.htm