Cryo-EM Analysis of Ribosome Structures at Creative Biostructure, Slides of Biology

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Ribosome Structure

Determination

The ribosome is a translational machinery that serves as the site of

protein synthesis. It is made from complexes of ribosomal RNAs

(rRNAs) and proteins which are arranged into two distinct

components of different sizes. The ribosome is one of the most

complex of protein/RNA, and obtaining the structure of the ribosome

has remained a challenge due to the atom complexity of ribosomes

and it is unstable because the aggregation and dissociation will

happen when the environmental conditions and physiological state

change.

At Creative Biostructure, we have the most advanced high-resolution

electron microscopy equipment and a direct electron detector in

combination with enhanced image processing, 3D reconstruction and

atomic model building. Our platform has the ability to analyze ribosome

structures from various species at the atomic level within their

physiological environments.

The working process:

Sample preparation: we have the advanced and mature technology of

protein expression and purification to generate ribosomes (also can provide

by customer).

Vitrification of samples: the sample must be kept at cryogenic

temperatures in order to minimize damage caused by the electron beam

and result in high quality images.

Image processing: Creative Biostructure utilizes the most advanced high-

resolution electron microscopy and enhanced image processing to generate

high resolution 2D images.

3D reconstruction and atomic model building: On the basis of high

resolution 2D images to produce a 3D structural model by using

computational procedures.

Analysis of structure.

Creative Biostructure has a professional team for experimental design

and Cryo-EM data analysis. We are dedicated to provide its customers

with high quality, satisfying and excellent service.

Excerpt from:

tps://www.creative-biostructure.com/Cryo-EM-for-Ribosomes-

597.htm