Cryo-EM Single Particle Analysis: A Powerful Tool for Structural Biology, Slides of Biology

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Cryo-EM Single

Particle Analysis

In recent years, cryo-electron microscopy (cryo-EM) technology has

made great progress, and it has been widely applied in the study of

high-precision structures of biological macromolecules, especially in

the structural analysis of protein complexes which are difficult to be

solved by other approaches. Cryo-EM single-particle analysis (SPA) has

become a popular and effective approach for studying the structure of

biologically significant molecules at a near-atomic resolution. As a

professional service provider in the field of structural biology, Creative

Biostructure has extensive expertise and experience in SPA

technology.

The Workflow of Our Cryo-EM SPA Service

Our cryo-EM SPA service covers the entire technical process. The

sample to be analyzed can be supplied by our customers or produced

by us, and then subjected to negative staining EM (optional). Negative

staining EM is a convenient approach for quickly screening

homogeneous samples and offers an initial model for acquiring cryo-EM

structures with high resolution. Followed by operations related to cryo-

EM SPA, including sample vitrification, EM data acquisition and

processing, three-dimensional (3D) model reconstruction and

refinement.

If your research project involves biological macromolecules or protein

complexes that are difficult to crystallize, then our cryo-EM SPA

service will effectively provide you with structural details about these

molecules. Please feel free to contact us for more information or a

detailed quote.

Excerpt from:

https://www.creative-biostructure.com/single-particle-analysis-

spa.htm