Cryo-EM: Revolutionizing DNA Visualization in Biology and Drug Design, Slides of Biology

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DNA Sample Cryo-EM

structure

Cryo-electron microscopy (cryo-EM) can be used to visualize DNA or

RNA structures based on our cryo-EM platform provided by Creative

Biostructure. We develop a novel suite of tools to overcome the size

and throughput limitations of cryo-EM, which can be applied to small

molecules such as DNA or RNA. It allows us to create soluble

nanostructures with an unprecedented combination of spatial

resolution and chemical versatility. In principle, we can attach any

moiety to our devices as long as it has a DNA binding domain or it can

be coupled to DNA.

Our research team has experience in exploiting cryo-EM image analysis

to obtain structural information to reveal the configuration of a

designed DNA nanostructure (Figure 1). We successfully obtained the

3D classification model of the DNA samples offered by our customer. All

the 2D TEM particles were classified by the 3D similarity and

consistence by using 3D classification function. For practical

applications, it is essential to verify that the 3D structures are

accurately produced as designed, which requires high resolution, at

least sufficient to resolve the DNA helix.

Reference

Takayuki Kato, Russell P. Goodman et al. (2009) High-Resolution

Structural Analysis of a DNA Nanostructure by cryoEM_. Nano Lett._ 2009,

9 (7), pp 2747–2750.