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An experiment using a water-drop projector to observe microorganisms in pond water. The physics behind the image formation in the paraxial region is explained using geometrical optics. The history of microscope observation is also discussed, including the invention by antoni van leeuwenhoek and the construction of a simple single-lens microscope using a water drop. Instructions for setting up the experiment and observing the magnified shadow images of various animals are provided.
Tipologia: Manuais, Projetos, Pesquisas
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Slovenia; [email protected]
ue to a growing interest in biology among science students, using demon- stration experiments in physics classes that in- volve observations of biological material or that relate to plant, animal, or human physiology can often raise student motivation. The experi- ment described below involves observation of microorganisms in a drop of pond water. An explanation of the image formation in the paraxial region is then given using geometrical optics. Beginning with the invention of the first mi- croscope, humans have been fascinated by the observation of microscopic life. In his spare time, Dutch draper Antoni van Leeuwenhoek (1632-1723) made his own single-lens micro- scope using a small glass sphere. Keeping de-
tailed records of his observations, he made the first drawing of a bacterium in 1683. Articles describing construction of a simple variation of Leeuwenhoek’s microscope (glass tubing micro- scope) have appeared previously in TPT. 1 However, it is easier still to build a single-lens microscope by using a drop of clear water in- stead of a glass lens. The small water drop works as a spherical lens with a large magnifying power.
To collect your sample, fill a syringe with water from a pond or large puddle with a lot of decaying plants. If you live at the coast, use sea- water. Try to catch some very small animals (0. mm to 0.5 mm) that move around in the water. Many of them are found close to the bottom of the pond. Fix the syringe on a holder (Lego blocks or a piece of wood works well) using adhesive tape as shown in Fig. 1. For a light source, fix a laser pointer on a vertical positioner. An empty stick-deodorant container will do, as shown in Fig. 1. To create your lens, carefully push the syringe piston until a water drop (about 2 mm in diameter) is formed at the end of the nozzle.
Place the syringe holder on a table about two meters away from a screen or white wall. Switch on the laser pointer and adjust the beam to point exactly through the middle of the water drop and perpendicular to the screen. For fine horizontal adjustment of the laser pointer, move the deodorant container, and for vertical adjust- ment use the built-in turn screw. With the right adjustment of the laser, a bright spot extends in- to a large round image on the screen about 2 m
Fig. 1. Water-drop projector: experiment setup.
in diameter. Now, if your pond water is rich enough with little animals, you should see their magnified shadow images floating and moving around on the screen. Small single-cell animals like paramecium appear as dark spots surround- ed with interference fringe contours. Some of them exhibit movements, but no detailed struc- ture can be recognized. Larger animals such as mosquito larva ( Anopheles species ), cyclops ( Cy- clops strenuus ), or water fleas ( Daphnia species ) appear like real monsters on the screen (see Fig. 2). Here you can clearly identify individual parts of their bodies and follow their move- ments. In our case the images were projected on the white wall and photographs were obtained with a digital camera (Kodak DC260). You may at first see nothing but the dark floating spots surrounded by a few concentric circles caused by light diffraction on small parts of de- caying plants and single-cell protozoa. Be pa- tient. The light attracts the little animals (a green laser works best), and after some time they swim down to the syringe nozzle and into the water drop. You can also give them a little help by dripping a few drops from the syringe.
The drop at the end of the syringe, though not a perfect sphere, can be treated as a small spherical lens. The light beam that falls on the drop refracts both times as it passes through the water-air interface. Let’s follow the path of the ray that enters the water drop just above an ob- ject that floats in the water drop at a small dis- tance x from the geometrical axis ( Fig. 3). The ray will refract twice and reach the screen at the distance y below the geometrical axis. The dis- tance y is determined by the distance from the drop to the screen d and the angle^ , which can be calculated using simple geometry:
= – RST
RST = 2 –
= 2( – ).
Using Snell's law,
Cyclops ( Cyclops strenuus )
Mosquito larva ( Anopheles species )
Water flea ( Daphnia species )
Fig. 2. Photographs of projected images. The actual size of each projected image is about 1 m x 1 m.
Author: are you certain of the taxonomic identity of this ani- mal? If not, then perhaps “Cyclops ( Cyclops species )” would be a more appropriate caption for first photo below.
jects to explore themselves. 2 However, there are other ways to make a water drop lens that is closer to a sphere than the one we suggest. For example, wrap a copper wire (thickness of 0. mm or so) to make a small ring, then touch the ring with the water drop hanging from the sy- ringe. The surface tension will make the drop jump onto the ring, where it will stay and form an almost perfect sphere. Or simply touch the drop from the syringe with a microscope glass plate and you’ll get a nice plan-concave lens. These alternatives can be used to observe the small animals in the pond water, and the ob- served shadow images may be even better than those obtained with the hanging water drop method. But the advantage of this method is the simple construction and the fact that you can have many animals inside the syringe. The little animals attracted by the light swim down into the drop by themselves. With the other methods, getting the animals into the drop may be difficult, and the observation time is limited by the evaporation of the drop.
References
Fig. 4. Water-drop magnification as the function of distance from the optical axis.