3D Cryo-EM for Antigen-Antibody Complex Structural Analysis at Creative Biostructure, Slides of Biology

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Antigen-antibody

Complex Structure

service

Creative Biostructure enables to advance 3D cryo-electron microscopy

(cryo-EM) into antigen-antibody complex applications for drug

discovery. 3D cryo-EM enables the structure determination of large

protein complexes like antigen-antibody complexes, or membrane

proteins. We expertise in protein production and proprietary expression

and cell culture tools, including antigen-antibody complexes, make

proteins of highest quality available. Your target of choice can be

produced at Creative Biostructure and subsequently analyzed with

cryo-EM for 3D reconstruction or epitope mapping applications.

Typical workflow comprises:

Generation of high quality antigen-antibody complex of the target of interest (performed

by Creative Biostructure or provided by the client).

Cryogenic sample preparation: Small amounts are prepared and flash-frozen to form an

amorphous ice phase that preserves native, solution-like conditions.

Electron microscope imaging: An advanced transmission electron microscope is used to

take high resolution 2D images of the vitrified sample in a low-exposure mode.

Computational 3D reconstruction: Iterative computational procedures are used to

aggregate the 2D images into a 3D structure. Several protocols like electron tomography,

single particle analysis, or sub-volume averaging are used.

Analysis and interpretation: The 3D models are verified and analyzed. Technological

advances are making atomic resolution of large proteins possible.

The workflow designed by our experts includes an analysis process and

a sample optimization system that automatically determines the buffer,

pH, and ligand conditions necessary to maintain optimal stability of the

sample during analysis. This represents a critical step prior to the

subsequent EM analysis, as it ensures its overall success. EM-based

structural analysis has emerged as an important complementary

technique to traditional methods, such as X-ray crystallography and

nuclear magnetic resonance (NMR).

There is a growing demand for structural studies from the

pharmaceutical industry, driven by the need for a deeper scientific

understanding, and also because of stronger regulatory constraints. In

the field of biologics, EM analysis has proven to be capable of delivering

critical information for antibody selection, epitope mapping and

formulation.

Excerpt from:

https://www.creative-biostructure.com/Cryo-EM-for-Antigen-

antibody-Complex-595.htm