Understanding Filament Structure through Cryo-EM at Creative Biostructure, Slides of Biology

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Filament Structure

Determination

Cryo-Electron Microscopy (Cryo-EM) techniques have been essential

for understanding the structure of biological specimens such as cells,

tissues and macromolecular complexes. Characterizing the molecular

structure of macromolecules is crucial for getting an insight into

understanding of the biochemical and cellular processes. Due to there

is not required crystallization and only small amounts of sample are

needed, Cryo-EM has become a major technique in structural biology

for the studies of functional complexes. Meanwhile, with the

advanced of image processing and three-dimensional reconstruction

techniques, Cryo-EM can also provide a three-dimensional (3D) maps

of biological macromolecules at a near-atomic resolutions.

At Creative Biostructure, our service includes the preparation and

purification of filaments, negative staining EM and image processing of

Cryo-EM, provide the customer with the 3D reconstruction at a near

atomic resolution, and finally provides a biologically insightful for

understanding the biological progress.

References

Eva Nogales, Sjors H.W. Scheres, Cryo-EM: A Unique Tool for the

Visualization of Macromolecular Complexity. Mol Cell, 2015; 58:677-

Lingyun Zhao, Jingfei Xu, et al. Determining the RAD51-DNA

Nucleoprotein Filament Structure and Function by Cryo-Electron

Microscopy. Methods Enzymol, 2018; 600:179-199.

Ahmet Mentesa, Andrew Huehn, et al. High-resolution cryo-EM

structures of actin-bound myosin states reveal the mechanism of

myosin force sensing. Proc Natl Acad Sci U S A. 2018; 115:1292-1297.