Small Protein
structure service
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Cryo-EM for Small Protein Structural Analysis: Services by Creative Biostructure, Slides of Biology
Cryo-electron microscopy (cryo-EM) is an advanced technique in structural biology used to study biological samples at cryogenic temperatures. Small proteins pose a challenge due to their difficulty in visualization, alignment, and validation. Creative Biostructure offers high-resolution imaging and three-dimensional reconstruction services for small proteins using improved sample preparation, selection of binding agents, optimization of electron dose, defocus, energy filter, and phase plate for contrast enhancement. The advantages include sample-saving, fast turnaround time, and cost-effectiveness. References: Merk A, et al. (2016) Cell165(7):1698-1707. Wu S, et al. (2012) Structure20(4):582-592.
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2020/2021
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Small Protein
structure service
Cryo-electron microscopy (cryo-EM) is a form of transmission electron
microscopy (TEM) where biological samples are studied at cryogenic
temperatures. It is becoming a more and more important technique in
the field of structural biology, as has been manifested by many once
hard-to-determine macromolecular structures. With the rapid
advancement of both hardware and software, the resolution of cryo-
EM maps is improving steadily. In some cases, near-atomic resolution
had been obtained, including those of viruses, ribosomes,
mitochondria, ion channels, and enzyme complexes as small as 150
kD.
Improved sample preparation with ultra-thin vitreous ice layer
Selection of binding agents such as Fabs
Optimization of the electron dose
Variation of defocus
Energy filter and correction of beam-induced motion
Use of phase plate for contrast enhancement
References:
Merk A, et al. (2016) “Breaking cryo-EM resolution barriers to facilitate
drug discovery”. Cell 165(7):1698-1707.
Wu S, et al. (2012) “Fabs enable single particle cryo-EM studies of small
proteins”. Structure 20(4):582-592.