Cryo-Electron Tomography
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Cryo-EM Tomography: Advanced 3D Visualization of Frozen Samples, Slides of Biology
Cryo-electron tomography (cryo-ET) is a cutting-edge technique used to visualize frozen hydrated biological samples without damaging their structure. This method uses an electron microscope to capture 2D projection images of a sample tilted at cryogenic temperatures. By aligning and combining these images mathematically, 3D reconstructions of the samples can be generated, providing a more comprehensive understanding of their morphology. Cryo-ET is particularly useful for examining intracellular organization of macromolecules and characterizing drug delivery vehicles. Creative Biostructure offers cryo-ET services with advanced instrumentation and expertise, committed to collaborating with clients to solve problems and advance cell biology and drug design.
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2019/2020
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Cryo-Electron Tomography
Cryo-electron tomography (cryo-ET) is a technique enabling the visualization of frozen hydrated biological samples intact from harmful preparation process. Cryo-ET uses an electron microscope to record a series of 2D projection images acquired by tilting a biological sample held at cryogenic temperature. Using computational methods, the 3D images can be aligned and combined mathematically to generate a 3D (tomographic) reconstruction of the sample. A more complete understanding of the particle morphology is accessible by extracting individual slices in different orientations. The 3D volume can also be examined to resolve any ambiguities of the 2D images. Our cryo-ET services lead the global market with unique advantages: 1.3D structure reconstruction at 2-5 Å resolution 2.Visualization of intracellular, undisturbed organization of macromolecules 3.State-of-the-art instrument 4.Versatile data analysis, including 2D suitability and averaging, and 3D reconstruction from existing data sets, and 3D structure refinement
Creative Biostructure is equipped with state-of-the-art electron microscopes and expertise in cryo-ET. We are always determined to work with our clients closely to solve problems, which will benefit the complete understanding of cell biology and drug design and development in the long run. Reference 1.Lučić, V.; et al. Cryo-electron tomography: The challenge of doing structural biology in situ. J Cell Biol Aug 2013, 202 (3) 407-419.