Baixe MicroNIR (Espectroscopia do Infravermelho Próximo) e outras Esquemas em PDF para Química Aplicada, somente na Docsity! Currently, sugarcane breeding programs evaluate sucrose content in their genotypes by a laborious methodology, in which the stalks need to be crushed. The MicroNIR OnSite-W can capture NIR spectral from stalks directly in the field and predict the sucrose content without destroying the biological material. OBJECTIVE CONCLUSION RESULTS AND DISCUSSION . ACKNOWLEDGMENTS MATERIALS AND METHODS This study aimed to construct a calibration curve to predict the sucrose content of juice (POL%J) using MicroNIR. NIR data were collected from 50 genotypes from the Brazilian Panel of Sugarcane Genotypes (BPSG) and also from genotypes from breeding trials of the Sugarcane Genetic Breeding Program of UFSCar, between 2020 and 2023 (Figure 1). As a reference method, conventional laboratory analysis was carried out to obtain POL%J values. UNSCRAMBLER (CAMO Technologies) and SPECTRAL SOFT (Spectral Solutions) software were used for data analysis. Different chemometric preprocessing was tested and standard normal variate (SNV) plus the first Savitzky-Golay derivative with 5-point window was selected. The outliers were excluded by applying the Mahalanobis distance with a 90% confidence interval. The calibration curve was constructed using a partial least squares (PLS) regression model. To ensure a good prediction, the data set was randomly divided into a calibration set (70% of the samples) and a cross-validation set (30% of the samples). In this study, we obtained 2,461 data points and 72.2% are on the calibration curve (Figure 2). The root mean square error of calibration (RMSEC) and the root mean square error of validation (RMSEV) were 0.93 and 0.95, respectively. The coefficients of determination for calibration (R2C) and cross-validation (R2V) were 0.92 and 0.91, respectively. The near-infrared spectroscopy can be used in the initial stages of sugarcane breeding programs to assist in the early selection of genotypes with high sucrose content. To the RIDESA/UFSCar team for the support on data collection, and Spectral Solutions for the support on discussions about data analysis. NEAR INFRARED SPECTROSCOPY FOR PREDICTING SUCROSE CONTENT IN SUGARCANE BREEDING TRIALS Gustavo Candido Jacobassi; Thiago Willian Almeida Balsalobre; Nathan Euclair Pinto; Roberto Giacomini Chapola; Hermann Paulo Hoffmann; Monalisa Sampaio Carneiro Sugarcane Genetic Breeding Program of the Federal University of São Carlos, Department of Biotechnology and Plant and Animal Production, Center for Agricultural Sciences, Araras-SP. E-mail:
[email protected] INTRODUCTION RMSEC: 0.92 RMSECV: 0.95 R²CV: 0.91 Min: 7.23 Max: 24.80 These results showed that the calibration curve for POL%J has a high correlation with the reference values and suggest that the prediction error from external validation may be acceptable. Figure 1. Collection of NIR spectra in samples from the Brazilian Panel of Sugarcane Genotypes (BPSG). Figure 2. Calibration curve for sucrose content of juice (POL%J) constructed using data collected by the RIDESA/UFSCar team and analyzed using UNSCRAMBLER and SPECTRAL SOFT software.
