we Placed a small drop of a negative stain on the slide. • we using sterile technique, then added a loopful of bacterial culture to slide, smearing it in the dye. • With an other slide, drag the ink-cell mixture into a thin film along the first slide and let stand for 5-1 minutes. • Allow to air dry (do not heat fix). • Flood the smear with crystal violet stain for about 1 minutes. The stain is gently washed with CuSO4 solution to get rid of excess crystal violet • Dry the contents of the slide • Scan the slide under the microscope the crystal violet by tilting the slide at a 45 degree angle and let stain run off until it air dries . • Examine the smear microscopically (100X